Effects of denervation on energy metabolism of rat hindlimb muscles: application of (31)P-MRS and (19)F-MRS

• Published in: Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association Vol. 4; no. 5; p. 370
• Main Authors: Miki, N, Ikata, T, Takai, H, Takata, S, Koga, K, Sogabe, T
• Format: Journal Article
• Language: English
• Published: Japan 1999
• Subjects: Animals; Blood Substitutes; Denervation; Electric Stimulation; Energy Metabolism; Fluorine Radioisotopes; Fluorocarbons; Follow-Up Studies; Hindlimb - blood supply; Hindlimb - innervation; Hindlimb - metabolism; Hydrogen-Ion Concentration; Isometric Contraction - physiology; Magnetic Resonance Spectroscopy; Male; Muscle, Skeletal - blood supply; Muscle, Skeletal - innervation; Muscle, Skeletal - metabolism; Muscular Atrophy - metabolism; Muscular Atrophy - pathology; Organ Size; Phosphates - analysis; Phosphocreatine - analysis; Phosphorus Isotopes; Physical Exertion - physiology; Radiopharmaceuticals; Rats; Rats, Wistar; Regional Blood Flow - physiology; Sciatic Nerve - physiology; Sciatic Nerve - surgery
• ISSN: 0949-2658
• DOI: 10.1007/s007760050118

We studied the effects of denervation on the energy metabolism and peripheral circulation dynamics of rat hindlimb muscles during and after exercise. The sciatic nerves of male Wistar rats were cut to produce denervation. Energy metabolism was assessed by phosphorus-31 magnetic resonance spectroscopy (MRS), and circulation by fluorine-19 MRS. Exercise of rat hindlimb muscles was induced by electrical stimulation at 40 Hz. The inorganic phosphate (Pi) / ¿Pi + phosphocreatine (PCr)¿ ratio, an indicator of the energy level, was 0.795 immediately after denervation. The ratios 4 and 8 weeks after denervation were 0.870 and 0.853, respectively. The intracellular pH during the 4 min after initiation of stimulation was significantly reduced 4 and 8 weeks after denervation compared with the value immediately after denervation. The signal strength of the research perfluoro-carbon (FC-43; perfluorotributylamine) a measure of circulation dynamics, increased to 167% in controls during exercise, but an increase of only 134% was seen in rats 8 weeks after denervation. These results showed that the energy supply and circulation dynamics in denervated atrophic muscles decreased during exercise compared with findings in control muscles.