Enhanced delivery of protein fused to cell penetrating peptides to mammalian cells

• Published in: BMB reports Vol. 52; no. 5; pp. 324 - 329
• Main Authors: Moon, Jung-Il, Han, Min-Joon, Yu, Shin-Hye, Lee, Eun-Hye, Kim, Sang-Mi, Han, Kyuboem, Park, Chang-Hwan, Kim, Chun-Hyung
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 31-05-2019
• Subjects: Amodiaquine (AQ); Cell Penetrating Peptide (CPP); Differentiation; Polylysine (9K); Reprogramming; Polylysine (9K); Cell Penetrating Peptide (CPP); Amodiaquine (AQ); Reprogramming; Differentiation
• ISSN: 1976-6696; 1976-670X

Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols. [BMB Reports 2019; 52(5): 324-329]