Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells

• Published in: BMB reports Vol. 42; no. 10; pp. 691 - 696
• Main Authors: Shen, Yan-Nan, Kim, Yoon-Mo, Yun, Cheol-Heui, Moon, Yang-Soo, Kim, Sang-Hoon
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 31-10-2009
• Subjects: Adipocyte; E2F1; Pref-1; Promoter; Transcriptional activation; Promoter; E2F1; Adipocyte; Transcriptional activation; Pref-1
• ISSN: 1976-6696; 1976-670X

The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.