Visualization of Candidate Division OP3 Cocci in Limonene-Degrading Methanogenic Cultures

Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific prob...

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Bibliographic Details
Published in:	Journal of microbiology and biotechnology Vol. 22; no. 4; pp. 457 - 461
Main Authors:	Rotaru, Amelia-Elena, Schauer, Regina, Probian, Christina, Mussmann, Marc, Harder, Jens
Format:	Journal Article
Language:	Korean
Published:	한국미생물생명공학회 30-04-2012
Subjects:	Candidate division OP3 hydrocarbon degradation Limonene methanogenesis monoterpene syntrophy limonene monoterpene hydrocarbon degradation methanogenesis syntrophy Candidate division OP3
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Summary:	Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific probes [OP3-565 and Eub-338(VI)] revealed abundant small OP3 cocci attached to larger cells. Syntrophic Deltaproteobacteria, OP3 cells, and methanogens affiliating with Methanoculleus and Methanosaeta formed the limonenedegrading community.
Bibliography:	The Korean Society for Applied Microbiology KISTI1.1003/JNL.JAKO201224237129437
ISSN:	1017-7825 1738-8872