Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase

• Published in: Journal of microbiology and biotechnology Vol. 29; no. 7; pp. 1043 - 1052
• Main Authors: Zhu, Jing, Liu, Yanjing, Yanqin, Yanqin, Pan, Lixia, Li, Yi, Liang, Ge, Wang, Qingyan
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 31-07-2019
• Subjects: alkaline lipase; biocatalysis; Burkholderia gladioli Bps-1; purification; Burkholderia gladioli Bps-1; alkaline lipase; purification; biocatalysis
• ISSN: 1017-7825; 1738-8872

Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with p-NPC 16 at a pH of 8.5 and 50°C, and the K m , k cat , and k cat /K m values were 1.05 mM, 292.95 s -1 and 279 s -1 mM -1 , respectively. The lipase was highly stable at 7.5 ≤ pH ≤ 10.0. K + and Na + exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.