STUDIES ON GASTRIC JUICE PROTEIN V. FRACTIONATION AND CHARACTERIZATION OF BIOLOGICALLY ACTIVE COMPONENTS IN GASTRIC JUICE

• Published in: GANN Japanese Journal of Cancer Research Vol. 52; no. 1; pp. 27 - 38
• Main Authors: WADA, TAKEO, SATO, KENSUKE, TAKAMURA, TAMIO, NISHIHARA, TAKEHARU, KONDO, MIYUKI, IBAYASHI, JUN, OHARA, HIROMICHI, ANZAI, TETSURO
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Cancer Association 01-03-1961
• Subjects: Chemical Fractionation; Dose Fractionation; Gastric Juice - chemistry; Humans; Old Medline; Proteins - chemistry; PROTEINS/chemistry; GASTRIC JUICE/chemistry
• ISSN: 0016-450X
• DOI: 10.20772/cancersci1959.52.1_27

1) Gastric juice of patients with carcinoma of the stomach was found to have low peptic activity. Non-carcinomatous histamine-refractory achlorhydric gastric juice after stimulation with insulin was found to be often accompanied with increase in pepsin. 2) Pepsin present in gastric juice was detected as a combination of two peaks when tested with continuous electrophoresis. The minor peak having an electrophoretic mobility of B2 was thought to be pepsinogen, whereas the major pepsin peak having a mobility faster than B1 was probably an interaction product between pepsin and gastric juice protein, according to the result of crossing electrophoresis. 3) Pepsin was found to appear in P-I fraction when gastric juice was subjected to cation exchange chromatography. 4) Carcinomatous gastric was found to contain increased amounts of blood group substances. This was not the result of simple contamination with bleeding. Cation exchange chromatography of gastric juice revealed that carcinomatous gastric juice was characterized by a specific increase of blood group activity in P-V. 5) Both continuous paper electrophoresis and cation exchange chromatography were available for complete separation of KIK factor and toxohormone. The significance of chemical change in corresponding fractions for the diagnosis of cancer was discussed.