HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) FOR THE DETERMINATION OF PANIPENEM/BETAMIPRON CONCENTRATIONS IN BODY FLUIDS

• Published in: CHEMOTHERAPY Vol. 39; no. Supplement3; pp. 197 - 205
• Main Authors: Hisaoka, Masafumi, Naganuma, Hideo, Yamazaki, Yasushi, Takahagi, Hidekuni, Kawahara, Yukinori
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Society of Chemotherapy 1991
• Subjects: Betamipron; Body fluids; HPLC; Panipenem; R976-2
• ISSN: 0009-3165; 1884-5894
• DOI: 10.11250/chemotherapy1953.39.Supplement3_197

High-performance liquid chromatography (HPLC) was developed for the determination of concentrations of panipenem/betamipron (PAPM/BP), combination of a new carbapenem antibiotics, panipenem (PAPM), and an anionic transport inhibitor, betamipron (BP), and R976-2, main metabolite of PAPM. And also, stabilities of PAPM and BP in samples prepared by mixing body fluids and 1M MOPS solution (3-(N-morpholino)-propanesulfonic acid, pH 7.0) equivalently, were studied. 1) PAPM concentrations in human plasma and urine were determined by HPLC method with simple pretreatment of assay samples. Recoveries from plasma or urine were more than 97% and C. V. values were less than 5.3%, suggesting good accuracy of this method. Determination limits were about 0.3μg/ml for plasma and about 1.0μg/ml for urine. 2) R976-2 concentrations in plasma and urine were determined by column switching HPLC method. Recoveries from plasma and urine were approximately 100% and C.V. values were less than 4.7%, suggesting good accuracy of the method. Determination limits were about 1μg/ml for plasma and about 5μg/ml for urine. 3) BP in human plasma and urine were determined by HPLC method after extraction or dilution. Recoveries were approximately 95% and C.V. values were less than 1.7%, suggesting good accuracy of this method. Detemination limits were about 0.2μg/ml for plasma, about 0.5μg/ml for urine and about 0.4μg/g for tissues. 4) PAPM in plasma sample was stable for 5 days at-20°C and 28 days at-80°C, and also that in urine sample was stable for 7 days at-20°C and 42 days at-80°C. And BP in plasma and urine samples was stable for 42 days at both temperatures of-20°C and-80°C.