ASSAY METHOD OF HBK IN BIOLOGICAL BODY FLUIDS: II. HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY METHOD

ASSAY METHOD OF HBK IN BIOLOGICAL BODY FLUIDS II. HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY METHOD

A procedure for the high-performance liquid chromatographic (HPLC) determination of HBK in serum (or plasma) urine, and bile is described using post-column derivatization with ο-phthalaldehyde. The technique involves extraction of HBK from biological samples by using a CM-Sephadex column and analysi...

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Bibliographic Details
Published in:CHEMOTHERAPY Vol. 34; no. Supplement1; pp. 82 - 86
Main Authors: KOMIYA, IZUMI, MITOMI, NAYU, NISHIO, MOTOHIRO
Format: Journal Article
Language:English
Japanese
Published: Japanese Society of Chemotherapy 1986
Online Access:Get full text
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Summary:A procedure for the high-performance liquid chromatographic (HPLC) determination of HBK in serum (or plasma) urine, and bile is described using post-column derivatization with ο-phthalaldehyde. The technique involves extraction of HBK from biological samples by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Dibekacin is used as an internal standard. The accuracy of this HPLC assay is 2.7-3.8% in human serum and 1.0-1.7% in human urine. The HPLC assay of HBK in human serum and human urine correlates well with the microbiological assay.
ISSN:0009-3165
1884-5894
DOI:10.11250/chemotherapy1953.34.Supplement1_82