Genetic diversity in unique indigenous mango accessions (Appemidi) of the Western Ghats for certain fruit characteristics

• Published in: Current science (Bangalore) Vol. 103; no. 2; pp. 199 - 207
• Main Authors: Vasugi, C., Dinesh, M. R., Sekar, K., Shivashankara, K. S., Padmakar, B., Ravishankar, K. V.
• Format: Journal Article
• Language: English
• Published: Current Science Association 25-07-2012
• Subjects: Alleles; Aroma; Colors; DNA; Fruits; Genetic diversity; Genetic relationships; Genotypes; Mangifera indica; Phenotypic traits; Pickling; RESEARCH COMMUNICATIONS; Sugars
• ISSN: 0011-3891

Mango is one of the choicest fruit crops of the tropical and subtropical regions in the world. Utilization of the conserved germplasm in breeding programmes requires precise information on the genetic relationships between the accessions. Considering the difficulties involved in the traditional divergence studies based on morphological characterization, microsatellites were successfully used for genetic diversity analysis of the indigenous 'Appemidi' type. Also, the major compounds that contribute to the unique aroma of these types were estimated. The materials used in the study consisted of 43 accessions and 14 SSRs developed at the Indian Institute of Horticultural Research, Bangalore. Analysis of sap volatiles was done using GCMS fitted with a DB-5 MS column using helium as the carrier gas. The analysis of 211 bands detected by the 14 Simple Sequence Repeats (SSRs) markers showed unambiguous discrimination of the 43 mango genotypes. The dendrogram resulted in the grouping of accessions into two major clusters, viz. cluster I with highly acidic types and cluster II with less acidic and high TSS group. The aroma of pickle type of mangoes is due to totally different type of terpenes as well as a completely different combination of monoterpenes.