Somatic hybridization of the Passiflora species, P. edulis f. flavicarpa Degener. and P. incarnata L

• Published in: Journal of experimental botany Vol. 46; no. 288; pp. 777 - 785
• Main Authors: Otoni, W.C., Blackhall, N.W., Vaz, F.B. d'Utra, Casali, V.W., Power, J.B., Davey, M.R.
• Format: Journal Article
• Language: English
• Published: Oxford OXFORD UNIVERSITY PRESS 01-07-1995; Oxford University Press
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Fruits; Fundamental and applied biological sciences. Psychology; Gels; Genetic hybridization; Genetics and breeding of economic plants; Greenhouses; Hybridity; Inbreeding; Interspecific and intergeneric hybridization, introgressions; Plant breeding: fundamental aspects and methodology; Plant morphology; Plants; Protoplasts; Research Papers; Seeds; Ploidy; Passifloraceae; Leucyl aminopeptidase; Enzyme; Isozyme; Aminopeptidases; Backcross; Passiflora edulis; Cell fusion; Fruit crop; Dicotyledones; Morphology; Protoplast; Angiospermae; Interspecific hybridization; RAPD markers; Hydrolases; Somatic hybrid; Spermatophyta; Proteinases
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/46.7.777

Somatic hybrid plants have been produced following electrofusion of mesophyll protoplasts of P. edulis f. flavicarpa and P. incarnata. These hybrids exhibited morphological characteristics intermediate between those of the two parental species. Further evidence of hybridity has been provided by isozyme analysis, ploidy determinations and molecular characterization (RAPD analysis). These somatic hybrid plants have been used in first generation backcrossing experiments with both P. edulis f. flavicarpa and P. incarnata; descriptions are provided of the floral and fruit characteristics, as well as seed production.