Studies on Analysis of Pesticide Residues in Foods (XX) Analytical Method for Chlorobenzilate, Chloropropylate Phenisobromolate and Tetradifon in Crops

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 17; no. 4; pp. 302 - 307_1
• Main Authors: FUKUHARA, Katsuharu, TAKEDA, Mitsuharu, UCHIYAMA, Mitsuru
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Society for Food Hygiene and Safety 05-08-1976
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.17.302

The improved analytical method for chlorobenzylate, chloropropylate, phenisobromolate and tetradifon by gas chromatography equipped with electron capture detector was established. The method consists of three procedures, extraction with the mixture of acetone and n-hexane, clean up by activated charcoal-Florisil column chromatography and the determination by gas chromatography. The pesticide on column was eluted with 40% n-hexane in benzene and 20% n-hexane in ether. Organochlorine pesticides were eluted in the first fraction and chlorobenzylate, chloropropylate, phenisobromolate and tetradifon were eluted in the second fraction. For gas chromatographic determination of these insecticides, three stationary phases, 2% DEGS+0.5% phosphoric acid, 5% OV-17 and 5% XE-60, were used. The efficiency of the analytical method was evaluated by using seven crops fortified with organochlorine pesticides (0.008-0.054ppm) and the insecticides (0.025-0.2ppm). The average recoveries of organochlorine pesticides ranged from 87.3% for dieldrin to 96.7 for DDT in lettuce, and also those of the insecticides were in the range of 80.0% for chlorobenzylate in plum to 97.3% for tetradifon in cucumber fruit.