An electrochemical biosensor based on moleculary imprinted polymer for the quantification of creatinine in human urine samples

• Published in: 2017 ISOCS/IEEE International Symposium on Olfaction and Electronic Nose (ISOEN) pp. 1 - 3
• Main Authors: Diouf, A., Motia, S., El Alami El Hassani, N., El Bari, N., Bouchikhi, B.
• Format: Conference Proceeding
• Language: English
• Published: IEEE 01-05-2017
• Subjects: Biology; biosensor; Biosensors; creatinine; Electrodes; Gold; human urine; Instruments; Iron; Molecular imprinted polymers; Polymers; screen-printed gold electrodes
• DOI: 10.1109/ISOEN.2017.7968919

In this work, we demonstrate the quantification of creatinine (Cre) in human urine by using a molecularly imprinted polymer sensors (MIP). For creatinine binding, a carboxylic polyvinyl chloride (PVC-COOH) layer was functionalized onto screen-printed gold electrodes (Au-SPE). Afterward, a solution of acrylamide and bisacrylamide is polymerized around Au-SPE/PVC-COOH/Cre. For control, a non-imprinted polymer was prepared similarly but without creatinine addition. The MIP morphology and retention properties were monitored, using three instrumental techniques: voltammetry (cyclic voltammetry and differential pulse voltammetry), electrochemical impedance spectroscopy, and UV-Visible spectrophotometry. The MIP didn't recognize analogues, such as urea and glucose. Additionally, the biosensor has proven effective for human urine creatinine determination. Partial Least Squares analysis revealed a good correlation between the biosensor results and the colorimetric method's. Good linearity in the range from 0.1 ng/mL to 1 μg/mL was obtained with a LOD of 0.016 ng/mL, a highly selective recognition ability, simplicity, miniaturisable and cheap.