Ve1-mediated resistance against Verticillium does not involve a hypersensitive response in Arabidopsis

• Published in: Molecular plant pathology Vol. 14; no. 7; pp. 719 - 727
• Main Authors: Zhang, Zhao, van Esse, H. Peter, van Damme, Mireille, Fradin, Emilie F., Liu, Chun-Ming, Thomma, Bart P. H. J.
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing Ltd 01-09-2013; Blackwell; John Wiley & Sons, Inc; John Wiley and Sons Inc
• Subjects: Agrobacterium - physiology; Arabidopsis; Arabidopsis - genetics; Arabidopsis - immunology; Arabidopsis - microbiology; Biological and medical sciences; cell-death; defense responses; disease resistance; Disease Resistance - immunology; ethylene-inducing xylanase; functional-analysis; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; gated ion-channel; gene family; Lycopersicon esculentum; Medical research; Nicotiana - microbiology; Original; Pathogens; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Leaves - immunology; Plant Leaves - microbiology; Plant Proteins - metabolism; Plants, Genetically Modified; Pseudomonas syringae - physiology; receptor-like proteins; rhynchosporium-secalis; Seedlings - metabolism; Seedlings - microbiology; Solanum lycopersicum - metabolism; tomato ve1; Verticillium; Verticillium - physiology; Verticillium dahliae; Fungi; Arabidopsis thaliana; Resistance; Cruciferae; Dicotyledones; Hypersensitivity; Angiospermae; Verticillium; Spermatophyta; Fungi Imperfecti; Experimental plant
• ISSN: 1464-6722; 1364-3703
• DOI: 10.1111/mpp.12042

Summary
The recognition of pathogen effectors by plant immune receptors leads to the activation of immune responses that often include a hypersensitive response (HR): rapid and localized host cell death surrounding the site of attempted pathogen ingress. We have demonstrated previously that the recognition of the Verticillium dahliae effector protein Ave1 by the tomato immune receptor Ve1 triggers an HR in tomato and tobacco. Furthermore, we have demonstrated that tomato Ve1 provides Verticillium resistance in Arabidopsis upon Ave1 recognition. In this study, we investigated whether the co‐expression of Ve1 and Ave1 in Arabidopsis results in an HR, which could facilitate a forward genetics screen. Surprisingly, we found that the co‐expression of Ve1 and Ave1 does not induce an HR in Arabidopsis. These results suggest that an HR may occur as a consequence of Ve1/Ave1‐induced immune signalling in tomato and tobacco, but is not absolutely required for Verticillium resistance.