Influence of 50-nm polystyrene particles in inducing cytotoxicity in mice co-injected with carbon tetrachloride, cisplatin, or paraquat

• Published in: Pharmazie Vol. 67; no. 8; p. 712
• Main Authors: Shimizu, Y, Isoda, K, Tezuka, E, Yufu, T, Nagai, Y, Ishida, I, Tezuka, M
• Format: Journal Article
• Language: English
• Published: Germany 01-08-2012
• Subjects: Alanine Transaminase - blood; Animals; Antineoplastic Agents - chemistry; Antineoplastic Agents - toxicity; Aspartate Aminotransferases - blood; Blood Urea Nitrogen; Carbon Tetrachloride - chemistry; Carbon Tetrachloride - toxicity; Chemical and Drug Induced Liver Injury - pathology; Cisplatin - chemistry; Cisplatin - toxicity; Herbicides - chemistry; Herbicides - toxicity; Liver - pathology; Male; Mice; Mice, Inbred BALB C; Nanoparticles - chemistry; Nanoparticles - toxicity; Paraquat - chemistry; Paraquat - toxicity; Particle Size; Pharmaceutical Vehicles; Polystyrenes - chemistry; Polystyrenes - toxicity
• ISSN: 0031-7144
• DOI: 10.1691/ph.2012.1823

The toxicity of nanomaterials has yet to be fully investigated. In particular, the interactions between nanomaterials and therapeutic drugs require further study. We investigated whether nano-sized polystyrene particles affect drug-induced toxicity. The particles, which are widely used industrially, had diameters of 50 (NPP50), 200 (NPP200) or 1000 (NPP1000) nm. The toxic chemicals tested were carbon tetrachloride, cisplatin (a popular anti-tumor agent), and a widely used herbicide, paraquat. Mice were treated intraperitoneally with either carbon tetrachloride (0.01 ml/kg), cisplatin (100 micromol/kg) or paraquat (50 mg/kg), with or without intravenous administration of polystyrene particles. All treatments in the absence of the nanoparticles were non-lethal and did not result in severe toxicity. However, when mice were injected with paraquat or cisplatin together with polystyrene particles, synergistic, enhanced toxicity was observed in mice injected with NPP50. These synergic effects were not observed in mice co-injected with NPP200 or NPP1000. These findings suggest that further evaluation of the interactions between polystyrene nano-particles and drugs is a critical prerequisite to the pharmaceutical application of nanotechnology.