Dysregulation of miR-31 and miR-375 expression is associated with clinical outcomes in oral carcinoma

• Published in: Oral diseases Vol. 20; no. 4; pp. 345 - 351
• Main Authors: Siow, MY, Karen Ng, LP, Vincent Chong, VK, Jamaludin, M, Abraham, MT, Abdul Rahman, ZA, Kallarakkal, TG, Yang, Y-H, Cheong, SC, Zain, RB
• Format: Journal Article
• Language: English
• Published: Denmark Blackwell Publishing Ltd 01-05-2014; Wiley Subscription Services, Inc
• Subjects: Adult; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Clinical outcomes; clinico-pathological parameters; Dentistry; Female; Gene expression; Gene Expression Regulation, Neoplastic; Humans; Male; microRNA profiling; MicroRNAs; MicroRNAs - genetics; Middle Aged; miR-31; miR-375; Mouth Neoplasms - genetics; Mouth Neoplasms - pathology; Oral cancer; oral squamous cell carcinoma; Tumorigenesis; miR-31; clinico-pathological parameters; oral squamous cell carcinoma; microRNA profiling; miR-375
• ISSN: 1354-523X; 1601-0825
• DOI: 10.1111/odi.12118

Objectives
To identify differentially expressed miRNA between oral squamous cell carcinoma (OSCC) and non‐cancer (NC) and to associate these with clinico‐pathological parameters.
Materials and methods
miRNA microarray profiling was utilized to obtain the expression profile of miRNAs in four OSCC and four NC samples. The expression of miR‐31 and miR‐375 was further validated in 26 OSCC and three NC samples using real‐time‐PCR. The association between miRNA expression and clinico‐pathological parameters was tested by univariate and multivariate analyses.
Results
Microarray profiling demonstrated that 15 and four miRNAs were up‐regulated and down‐regulated, respectively, in OSCC as compared with NC. miR‐31 and miR‐375 were validated as up‐ and down‐regulated miRNAs, respectively. In univariate analyses, expression of miR‐31 was significantly elevated in early stage, tumours with no metastatic nodes and those from the buccal mucosa. By contrast, low miR‐375 expression was significantly associated with late stage disease, larger tumour size and the non‐cohesive type of pattern of invasion in OSCC. The association between miR‐31 expression with tumour staging and site and miR‐375 with tumour staging remained significant in multivariate analyses.
Conclusions
This study has identified 19 miRNAs significantly associated with OSCC, and expressions of miR‐31 and miR‐375 were significantly related with clinico‐pathological parameters suggesting they could be important in driving oral tumourigenesis.