Studies on biochemical markers in cryoinfarction in rats

• Published in: Cryo-Letters Vol. 27; no. 5; p. 311
• Main Authors: Hemalatha, Thiagarajan, Mala, Vairakkannu Vaijayanthi, Manohar, Bhakthavatsalam Murali, Nayeem, Mohammed, Subramaniam, Samu, Puvanakrishnan, Rengarajulu
• Format: Journal Article
• Language: English
• Published: England 01-09-2006
• Subjects: Animals; Biomarkers - blood; Cicatrix - pathology; Cold Temperature; Collagen - metabolism; Creatine Kinase, MB Form - blood; Electrocardiography; Fibrosis; Inflammation - pathology; Male; Myocardial Infarction - diagnosis; Myocardial Infarction - etiology; Myocardial Infarction - metabolism; Myocardial Infarction - pathology; Myocardium - metabolism; Myocardium - pathology; Myocytes, Cardiac - pathology; Natriuretic Peptide, Brain - blood; Peptide Fragments - blood; Rats; Rats, Wistar; Time Factors; Troponin T - blood
• ISSN: 0143-2044

Cryoinfarction in rats was carried out by placing the cooled probe directly on the midway of left anterior descending coronary artery. This caused damage to the blood vessel, hindering blood flow to the distal part of the left ventricle. Gross pathology showed around 26% infarction at 24 h. Histopathology revealed death of cardiomyocytes with blood vessel congestion at the end of 24 h, inflammatory infiltrate at 48 h, fibrotic scar by 96 h and collagen deposition by 192 h. Acute myocardial infarction biomarkers such as Cardiac Troponin T, Creatine Kinase MB and NT pro BNP were shown to be elevated by 4 h. ECG showed an ST segment elevation by 96 h. This cryoinfarction model was suitable to study changes taking place during acute myocardial infarction in humans.