Supercritical CO2 Assisted Extraction and LC-MS Identification of Picroside I and Picroside II from Picrorhiza kurroa

• Published in: Phytochemical analysis Vol. 24; no. 2; pp. 97 - 104
• Main Authors: Patil, Ajit A., Sachin, Bhusari S., Shinde, Devanand B., Wakte, Pravin S.
• Format: Journal Article
• Language: English
• Published: Bognor Regis Blackwell Publishing Ltd 01-02-2013; Wiley Subscription Services, Inc
• Subjects: Picrorrhiza kurroa; picroside I; picroside II; Supercritical carbon dioxide
• ISSN: 0958-0344; 1099-1565
• DOI: 10.1002/pca.2383

ABSTRACT
Introduction
Picroside I and picroside II have been studied intensively because of their pharmacological actions and clinical applications. Numerous methods have been reported for extracting picroside I and picroside II from Picrorrhiza. kurroa rhizomes. This is the first report of picroside I and picroside II extraction using the supercritical carbon dioxide assisted extraction technique.
Objective
To develop supercritical carbon dioxide assisted extraction and LC–MS identification of picroside I and picroside II from the Picrorrhiza kurroa Royle rhizomes.
Methodology
Surface response methodology based on 33 fractional factorial design was used to extract picroside I and picroside II from P. kurroa rhizomes. The effects of various process factors, namely temperature (40–80°C), pressure (25–35 MPa) and co‐solvent (methanol) concentration (0–10% v/v) on extraction yield of the two compounds were evaluated. The picroside I and picroside II contents were determined using validated LC–MS methodology.
Results
The maximum yield of picroside I (32.502 ± 1.131 mg/g) and picroside II (9.717 ± 0.382 mg/g) was obtained at the 10% v/v co‐solvent concentration, 40°C temperature and 30 MPa pressure. The conventional Soxhlet assisted methanol extract of P. kurroa powder resulted in 36.743 ± 1.75 and 11.251 ± 0.54 mg/g yield of picroside I and picroside II, respectively.
Conclusion
Variation of concentration and extraction time showed a significant effect on the picroside I and picroside II yield. Supercritical carbon dioxide assisted extraction using methanol as a co‐solvent is an efficient and environmentally sustainable method for extracting picroside I and picroside II from P. kurroa rhizomes. Copyright © 2012 John Wiley & Sons, Ltd.
The response surface methodology using 33 fractional factorial design was established to describe supercritical carbon dioxide assisted extraction of picroside I and II from the Picrorrhiza kurroa. The quantitative effects of extraction pressure, temperature and modifier concentration on yield of picroside were investigated. The maximum yield of picroside I (32.502 ± 1.131 mg/g) and picroside II (9.717 ± 0.382 mg/g) was obtained at the 10 % v/v co‐solvent concentration, 40 °C temperature and 30 MPa pressure.