Continuous production of carcinoembryonic antigen in hollow fiber cell culture units: brief communication

A line of cultured cells derived from a primary human adenocarcinoma of the colon was grown in the extracapillary spaces of hollow fiber tissue culture units in the absence of serum components of high molecular weight (greater than 10,000). The cells were capable of producing more than 30 microgram...

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Bibliographic Details
Published in:	JNCI : Journal of the National Cancer Institute Vol. 60; no. 2; p. 303
Main Authors:	David, G S, Reisfeld, R A, Chino, T H
Format:	Journal Article
Language:	English
Published:	United States 01-02-1978
Subjects:	Adenocarcinoma - immunology Carcinoembryonic Antigen Cell Line Colonic Neoplasms - immunology Culture Techniques - methods Humans Neoplasms, Experimental - immunology
Online Access:	Get more information
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Summary:	A line of cultured cells derived from a primary human adenocarcinoma of the colon was grown in the extracapillary spaces of hollow fiber tissue culture units in the absence of serum components of high molecular weight (greater than 10,000). The cells were capable of producing more than 30 microgram of carcinoembryonic antigen (CEA) per day, provided that the extracapillary fluid was changed frequently. The concanavalin-A binding and molecular sieve chromatography properties of the tissue culture-derived CEA were similar to those of CEA isolated from metastatic colon cancer tissue.
ISSN:	0027-8874
DOI:	10.1093/jnci/60.2.303