Carboxymethyl chitosan-methacrylic acid gelatin hydrogel for wound healing and vascular regeneration

• Published in: Biomedical materials (Bristol) Vol. 19; no. 4
• Main Authors: Yu, Jingrong, Gao, Zhengkun, Han, Qingyue, Wang, Zi, Zhang, Wenjie, Zhao, Jie, Qiao, Shan, Zou, Xinxin, Huang, Fengjie
• Format: Journal Article
• Language: English
• Published: England 18-06-2024
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bandages; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Chitosan - analogs & derivatives; Chitosan - chemistry; Cross-Linking Reagents - chemistry; Gelatin - chemistry; Hemostatics - chemistry; Hemostatics - pharmacology; Humans; Hydrogels - chemistry; Male; Materials Testing; Methacrylates - chemistry; Mice; Neovascularization, Physiologic - drug effects; Polyethylene Glycols - chemistry; Rats; Regeneration - drug effects; Wound Healing - drug effects; vascular regeneration; wound healing; methacrylic acid gelatin; carboxymethyl chitosan; hydrogel
• ISSN: 1748-605X
• DOI: 10.1088/1748-605X/ad5482

At present, wound dressings in clinical applications are primarily used for superficial skin wounds. However, these dressings have significant limitations, including poor biocompatibility and limited ability to promote wound healing. To address the issue, this study used aldehyde polyethylene glycol as the cross-linking agent to design a carboxymethyl chitosan-methacrylic acid gelatin hydrogel with enhanced biocompatibility, which can promote wound healing and angiogenesis. The CSDG hydrogel exhibits acid sensitivity, with a swelling ratio of up to 300%. Additionally, it exhibited excellent resistance to external stress, withstanding pressures of up to 160 kPa and self-deformation of 80%. Compared to commercially available chitosan wound gels, the CSDG hydrogel demonstrates excellent biocompatibility, antibacterial properties, and hemostatic ability. Both
and
results showed that the CSDG hydrogel accelerated blood vessel regeneration by upregulating the expression of CD31, IL-6, FGF, and VEGF, thereby promoting rapid healing of wounds. In conclusion, this study successfully prepared the CSDG hydrogel wound dressings, providing a new approach and method for the development of hydrogel dressings based on natural macromolecules.