Tissue response during staining and illumination of voltage-sensitive dye in rabbit myocardium

• Published in: 2007 Computers in Cardiology pp. 665 - 668
• Main Authors: Novakova, M., Nogova, K., Bardonova, J., Provaznik, I.
• Format: Conference Proceeding
• Language: English
• Published: IEEE 01-09-2007
• Subjects: Bleaching; Electron microscopy; Fluorescence; Heart; Lighting; Myocardium; Optical recording; Rabbits; Resistance; Voltage
• ISBN: 1424425336; 9781424425334
• ISSN: 0276-6574; 2325-8853
• DOI: 10.1109/CIC.2007.4745573

Voltage-sensitive dyes (VSDpsilas) undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field and are therefore used for recording of monophasic action potentials (MAPs) by optical method. S.-c. phototoxic effects of VSDs appear under intense illumination and include alterations in heart electrical signals. To examine putative side effects of VSD di-4-ANEPPS in rabbit hearts, we studied electrogram, mean coronary flow and formation of hydroxyl radicals in the control, staining and washout periods and during illumination. Possible ultrastructural changes were examined by electron microscopy. Rabbit myocardium is resistant to di-4-ANEPPS side effects during staining and also resistant to bleaching during intense illumination since only minor changes in ECG and no change in other parameters were observed.