On-chip microfluidics for proteomic analysis by electrospray ionization/mass spectrometry

• Published in: 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.00EX451) pp. 439 - 444
• Main Authors: Tabourier, P., Druon, C., Rolando, C., Lefebvre, P.
• Format: Conference Proceeding
• Language: English
• Published: IEEE 2000
• Subjects: Ionization; Mass spectroscopy; Microfluidics; Micropumps; Protein engineering; Proteomics; Prototypes; Silicon; Solids; Testing
• ISBN: 9780780366039; 0780366034
• DOI: 10.1109/MMB.2000.893823

Describes the development of a silicon integrated demonstrator coupled to an ElectroSpray Ionization source in view of protein analysis by Mass Spectrometry (ESI/MS). The design and the making of basic components: (i) a novel thermal valveless micropump, (ii) a Solid Phase Extraction (SPE) column and (iii) a hollow cantilever nanoemitter constitute the steps of the authors' ongoing technological program which will be achieved by their gathering within a single chip. At present a manifold prototype at a 10 /spl mu/m scale has been fabricated and is currently being tested.