Two-photon fluorescence resonance energy transfer stoichiometry in living cells

Two-photon fluorescence resonance energy transfer stoichiometry in living cells

Using phase-shaped pulses, we perform, for the first time, a proof-of-principle demonstration of two-photon fluorescence resonance energy transfer (FRET) microscopy for studying the stoichiometry of intermolecular interactions within living cells.

Saved in:
Bibliographic Details
Published in:2014 Conference on Lasers and Electro-Optics (CLEO) - Laser Science to Photonic Applications pp. 1 - 2
Main Authors: Bhagwat, Amar R., Flynn, Daniel, Brenner, Meredith H., Nunez, Marcos, Ogilvie, Jennifer P., Dawen Cai, Swanson, Joel A.
Format: Conference Proceeding
Language:English
Published: The Optical Society 01-06-2014
Subjects:
Cells (biology)
Educational institutions
Energy exchange
Fluorescence
Laser excitation
Microscopy
Optical filters
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Using phase-shaped pulses, we perform, for the first time, a proof-of-principle demonstration of two-photon fluorescence resonance energy transfer (FRET) microscopy for studying the stoichiometry of intermolecular interactions within living cells.
ISSN:2160-8989
2160-9004
DOI:10.1364/CLEO_AT.2014.ATh3P.2