Interleukin 1beta (IL-1beta), IL-10, tumor necrosis factor-alpha, and cellular proliferation index in peripheral blood mononuclear cells in patients with ankylosing spondylitis

• Published in: Journal of rheumatology Vol. 29; no. 3; pp. 522 - 526
• Main Authors: Mercado Monica Vazquez-Del, Araceli Garcia-Gonzalez, José Francisco Muñoz-Valle, Trinidad Garcia-Iglesias, Gloria Martinez-Bonilla, Guislaine Bernard-Medina, Adriana Sanchez-Ortiz, José M Ornelas-Aguirre, Mario Salazar-Paramo, Jorge I Gamez-Nava, Laura Gonzalez-Lopez
• Format: Journal Article
• Language: English
• Published: Canada The Journal of Rheumatology 01-03-2002
• Subjects: Adolescent; Adult; Cell Division - immunology; Cross-Sectional Studies; Cytokines - analysis; Humans; Interleukin-1 - analysis; Interleukin-10 - analysis; Leukocytes, Mononuclear - chemistry; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - immunology; Middle Aged; Severity of Illness Index; Spondylitis, Ankylosing - immunology; Tumor Necrosis Factor-alpha - analysis
• ISSN: 0315-162X; 1499-2752

OBJECTIVE: To evaluate cytokine production and cellular proliferation index (CPI) in peripheral blood mononuclear cells (PBMC)
of patients with ankylosing spondylitis (AS), and their association with clinical variables. METHODS: In a cross sectional
study we compared the production of tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), and IL-10 and CPI
in response to phytohemagglutinin (PHA) in PBMC of 27 patients with AS and 24 healthy controls. We also assessed clinical
characteristics including disease activity index (BASDAI) and functional index (BASFI). RESULTS: Levels of IL-1beta were higher
in patients with AS (median 242 pg/ml) than in controls (median 65 pg/ml); p = 0.002. No differences were observed in median
levels of TNF-alpha or IL-10 between AS and controls. Patients had a reduction in CPI (1.2 in AS vs 1.8 in controls; p < 0.001).
A positive correlation was observed between IL-10 production and age (rho = 0.34, p = 0.01). A borderline negative correlation
was observed between CPI and age (rho = -0.26, p = 0.07). CONCLUSION: Patients with AS had high production of IL-1beta compared
with controls and a poor response in CPI. These findings may explain the lack of response for microbial antigens mediated
by the innate immune response.