Frequent Methylation of Vimentin in Well-differentiated Gastric Carcinoma

• Published in: Anticancer research Vol. 29; no. 6; pp. 2227 - 2229
• Main Authors: KITAMURA, Yo-Hei, SHIRAHATA, Atsushi, HIBI, Kenji, SAKATA, Makiko, GOTO, Tetsuhiro, MIZUKAMI, Hiroki, SAITO, Mitsuo, ISHIBASHI, Kazuyoshi, KIGAWA, Gaku, NEMOTO, Hiroshi, SANADA, Yutaka
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-06-2009
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - secondary; Aged; Biological and medical sciences; Cell Differentiation; DNA Methylation; Female; Gastric Mucosa - metabolism; Gastric Mucosa - pathology; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression Regulation, Neoplastic; Humans; Lymphatic Metastasis; Male; Medical sciences; Neoplasm Staging; Polymerase Chain Reaction; Prognosis; Promoter Regions, Genetic; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Vimentin - genetics; Vimentin; Malignant tumor; Stomach cancer; quantitative methylation-specific PCR; Polymerase chain reaction; Stomach carcinoma; Cancerology; Digestive diseases; Frequency; Molecular biology; Methylation; gastric carcinoma; Cancer; Gastric disease; Quantitative analysis
• ISSN: 0250-7005; 1791-7530

Background: Recently, it was shown that the vimentin gene, usually activated in mesenchymal cells, was highly methylated in
colorectal carcinoma. Materials and Methods: The methylation status of the vimentin gene was examined in primary carcinomas
and the corresponding normal tissues derived from 37 patients with gastric carcinoma using quantitative methylation-specific
PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. Results.
Aberrant methylation of the vimentin gene was detected in 14 out of 37 (38%) primary gastric carcinomas. This result suggested
that the aberrant methylation of the vimentin gene was frequent in gastric carcinomas. Subsequently, clinicopathological data
were correlated with the methylation score. A significant difference was observed in histology (p=0.0429). In addition, a
trend was shown toward advancement of gastric carcinomas with vimentin methylation (p=0.0588). Conclusion: In gastric carcinomas,
well-differentiated adenocarcinoma was significantly methylated compared to poorly differentiated.