Molecular cloning of a novel human leukemia-associated gene. Evidence of conservation in animal species
We have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemic blast cells (myeloid and lymphoid) than in resting or proliferating nonleukemic lymphoid cells or chronic lymphoid and myeloid leukemic cells. In this report we describe the clon...
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| Published in: | The Journal of biological chemistry Vol. 264; no. 24; pp. 14556 - 14560 |
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| Main Authors: | , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
25-08-1989
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| Abstract | We have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemic
blast cells (myeloid and lymphoid) than in resting or proliferating nonleukemic lymphoid cells or chronic lymphoid and myeloid
leukemic cells. In this report we describe the cloning of two different sized full-length cDNAs that code for p18. The two
cDNAs differ in their 3'-noncoding regions as a result of alternative polyadenylation. Analysis of the complete nucleotide
sequence and the corresponding amino acid sequence did not reveal significant homology to any previously described sequences.
We show evidence that this gene is highly conserved in several animal species and low stringency hybridization studies suggest
that the p18 gene may be a member of a family of partially homologous genes in the human genome. |
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| AbstractList | We have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemic blast cells (myeloid and lymphoid) than in resting or proliferating nonleukemic lymphoid cells or chronic lymphoid and myeloid leukemic cells. In this report we describe the cloning of two different sized full-length cDNAs that code for p18. The two cDNAs differ in their 3'-noncoding regions as a result of alternative polyadenylation. Analysis of the complete nucleotide sequence and the corresponding amino acid sequence did not reveal significant homology to any previously described sequences. We show evidence that this gene is highly conserved in several animal species and low stringency hybridization studies suggest that the p18 gene may be a member of a family of partially homologous genes in the human genome. We have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemic blast cells (myeloid and lymphoid) than in resting or proliferating nonleukemic lymphoid cells or chronic lymphoid and myeloid leukemic cells. In this report we describe the cloning of two different sized full-length cDNAs that code for p18. The two cDNAs differ in their 3'-noncoding regions as a result of alternative polyadenylation. Analysis of the complete nucleotide sequence and the corresponding amino acid sequence did not reveal significant homology to any previously described sequences. We show evidence that this gene is highly conserved in several animal species and low stringency hybridization studies suggest that the p18 gene may be a member of a family of partially homologous genes in the human genome. The authors have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemia blast cells (myeloid and lymphoid) than in resting or proliferating nonleukemic lymphoid cells or chronic lymphoid and myeloid leukemic cells. In this report they describe the cloning of two different sized full-length cDNAs that code for p18. The two cDNAs differ in their 3'-noncoding regions as a result of alternative polyadenylation. Analysis of the complete nucleotide sequence and the corresponding amino acid sequence did not reveal significant homology to any previously described sequences. |
| Author | X X Zhu D A Fox R Melhem K Kozarsky F Lottspeich S M Hanash J Lowe J R Strahler G F Atweh C Eckerskorn |
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| Snippet | We have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemic
blast cells (myeloid and lymphoid)... We have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemic blast cells (myeloid and lymphoid)... The authors have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemia blast cells (myeloid and... |
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| SubjectTerms | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cattle Cell Line Chlorocebus aethiops Cloning, Molecular Cricetinae DNA - isolation & purification Dogs Ducks Fundamental and applied biological sciences. Psychology genes Genes. Genome HeLa Cells Humans Leukemia - genetics man Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data Neoplasm Proteins - genetics Neoplasm Proteins - isolation & purification Oncogenes Peptides - genetics Peptides - isolation & purification Swine |
| Title | Molecular cloning of a novel human leukemia-associated gene. Evidence of conservation in animal species |
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