Molecular cloning of a novel human leukemia-associated gene. Evidence of conservation in animal species

• Published in: The Journal of biological chemistry Vol. 264; no. 24; pp. 14556 - 14560
• Main Authors: XIAO-XIANG ZHU, KOZARSKY, K, STRAHLER, J. R, ECKERSKORN, C, LOTTSPEICH, F, MELHEM, R, LOWE, J, FOX, D. A, HANASH, S. M, ATWEH, G. F
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 25-08-1989
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cattle; Cell Line; Chlorocebus aethiops; Cloning, Molecular; Cricetinae; DNA - isolation & purification; Dogs; Ducks; Fundamental and applied biological sciences. Psychology; genes; Genes. Genome; HeLa Cells; Humans; Leukemia - genetics; man; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Neoplasm Proteins - genetics; Neoplasm Proteins - isolation & purification; Oncogenes; Peptides - genetics; Peptides - isolation & purification; Swine
• ISSN: 0021-9258; 1083-351X

We have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemic
blast cells (myeloid and lymphoid) than in resting or proliferating nonleukemic lymphoid cells or chronic lymphoid and myeloid
leukemic cells. In this report we describe the cloning of two different sized full-length cDNAs that code for p18. The two
cDNAs differ in their 3'-noncoding regions as a result of alternative polyadenylation. Analysis of the complete nucleotide
sequence and the corresponding amino acid sequence did not reveal significant homology to any previously described sequences.
We show evidence that this gene is highly conserved in several animal species and low stringency hybridization studies suggest
that the p18 gene may be a member of a family of partially homologous genes in the human genome.