Misexpression of the RNA-binding protein ELRB in Xenopus presumptive neurectoderm induces proliferation arrest and programmed cell death

• Published in: The International journal of developmental biology Vol. 43; no. 4; pp. 295 - 303
• Main Authors: Perron, M, Furrer, M P, Wegnez, M, Théodore, L
• Format: Journal Article
• Language: English
• Published: Spain University of the Basque Country Press 01-07-1999
• Subjects: Animals; Apoptosis; Apoptosis - genetics; Biological Markers; Biomarkers; Cell Division; Cell Division - genetics; Gene Expression Regulation, Developmental; In Situ Hybridization; In Vitro Techniques; Life Sciences; Nervous System; Nervous System - cytology; Nervous System - embryology; Neurons and Cognition; RNA-Binding Proteins; RNA-Binding Proteins - genetics; RNA-Binding Proteins - physiology; Xenopus; Xenopus - embryology; Xenopus - genetics
• ISSN: 0214-6282

Proteins of the ELAV/Hu family share the presence of three RNA binding domains. In Xenopus, three nervous system-specific elav/Hu related genes, elrB, elrC and elrD, have been identified so far. The temporally regulated expression patterns of elrB, elrC and elrD suggest their involvement at different steps of neural differentiation. In the present study we misexpressed elrB by RNA injection in early Xenopus embryos and analyzed morphologically and molecularly its effects on neural development. We showed that heterochronous expression of elrB in presumptive neurectoderm down-regulates the expression of neural markers, such as N-tubulin, as well as that of other Xenopus elav-like genes, elrC and elrD, whereas ectopic expression of elrB in presumptive mesoderm has no effect on MyoD. Misexpression of elrB also induces severe defects in neural tube development, associated with massive cell loss resulting from early cell cycle arrest and programmed cell death. Our results are discussed in the context of early neural differentiation.