Cytotoxic Effects of Treosulfan on Prostate Cancer Cell Lines

• Published in: Anticancer research Vol. 27; no. 4B; pp. 2403 - 2408
• Main Authors: FEYERABEND, Susan, FEIL, Gerhard, KRUG, Jutta, KASSEN, Annette, STENZL, Arnulf
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-07-2007
• Subjects: Antineoplastic Agents, Alkylating - pharmacology; Biological and medical sciences; Busulfan - analogs & derivatives; Busulfan - pharmacology; Cell Growth Processes - drug effects; Cell Line, Tumor; Cell Survival - drug effects; Dose-Response Relationship, Drug; Humans; Male; Medical sciences; Neoplasms, Hormone-Dependent - drug therapy; Neoplasms, Hormone-Dependent - pathology; Nephrology. Urinary tract diseases; Prostatic Neoplasms - drug therapy; Prostatic Neoplasms - pathology; Tumors; Tumors of the urinary system; Urinary tract. Prostate gland; Antineoplastic agent; Prostate carcinoma; PC3; Urinary system disease; LNCaP; Prostate disease; DU145; Alkanediol; Cytotoxicity; Malignant tumor; Treosulfan; Prostate adenocarcinoma; Alkanesulfonic acid; Alkylating agent; Cancerology; Established cell line; Male genital diseases; Prostate cancer; Tumor cell
• ISSN: 0250-7005; 1791-7530

Objective: Despite various therapeutical options in metastatic prostate cancer, the lack of a curative approach motivates
further investigations. Treosulfan is an alkylating agent that has proven its indication in the treatment of e.g. ovarian
carcinoma. This study focused on the objective of evaluating the effect of in vitro intoxication of human prostate carcinoma
cell lines with treosulfan. Materials and Methods: Human prostate cancer cell lines LNCaP, DU145 and PC3 were treated with
treosulfan concentrations from 0.5-500 μM for up to six days. Analysis of cell viability was performed using colorimetric
WST-1 assay. Control data were obtained from identical cell lines cultivated without treosulfan. Results: Incubation with
treosulfan inhibited cell viability and led to cell death in all cell lines in a dose- and time-dependent manner. After one
day, viability of LNCaP, DU145 and PC3 cells was constantly reduced with a dose rate of at least 10 μM (p<0.001), 10 μM (p<0.0001)
and 100 μM (p<0.0001) treosulfan, respectively. Minimum dose rates leading to death of nearly all LNCaP, DU145 and PC3 cells
were 250 μM, 100 μM and 200 μM treosulfan, respectively. Conclusion: The results demonstrate a sensitivity of prostate carcinoma
cells to the cytotoxic activity of treosulfan. Therefore, treosulfan might be a promising compound for novel treatment protocols
for prostate cancer.