Association between Allelic Polymorphisms of Metabolizing Enzymes (CYP 1A1, CYP 1A2, CYP 2E1, mEH) and Occurrence of Colorectal Cancer in Hungary

• Published in: Anticancer research Vol. 27; no. 4C; pp. 2931 - 2937
• Main Authors: KISS, Istvan, ORSOS, Zsuzsa, EMBER, Istvan, GOMBOS, Katalin, BOGNER, Barna, CSEJTEI, Andras, TIBOLD, Antal, VARGA, Zsuzsa, PAZSIT, Emese, MAGDA, Ingrid, ZOLYOMI, Annamaria
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-07-2007
• Subjects: Alleles; Biological and medical sciences; Case-Control Studies; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - genetics; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Epoxide Hydrolases - genetics; Epoxide Hydrolases - metabolism; Female; Gastroenterology. Liver. Pancreas. Abdomen; Genetic Predisposition to Disease; Humans; Hungary; Isoenzymes - genetics; Isoenzymes - metabolism; Male; Medical sciences; Middle Aged; Polymorphism, Genetic; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Hungary; Europe; Rectal disease; Genetic variability; Enzyme; Colorectal cancer; Genotype; Malignant tumor; CYP 1A2; Linkage equilibrium; CYP 1A1; Epoxide hydrolase; CYP 2E1; Colonic disease; mEH; Cancerology; Ether hydrolases; Digestive diseases; Hydrolases; Intestinal disease; genetic polymorphism; Polymorphism
• ISSN: 0250-7005; 1791-7530

Background: Genetic polymorphisms of metabolizing enzymes may affect the risk of cancer formation in humans. Since the diet
can contain polycyclic aromatic hydrocarbons (PAHs) and heterocyclic amines (HAs), the relationship between polymorphisms
of enzymes involved in PAH and HA metabolism and the occurrence of sporadic colorectal cancer was studied. Patients and Methods:
Five hundred colorectal cancer patients and 500 controls were genotyped for cytochrome P450 enzymes (CYP) 1A1 Ile/Val, CYP
1A2*1F, CYP 2E1 c1/c2, microsomal epoxy hydrolase (mEH) exon 3 Tyr113His and exon 4 His139Arg polymorphisms by allele-specific
polymerase chain reaction (PCR) or PCR-restriction fragmenth length polymorphism (RFLP). Results: The presence of CYP 1A1
Val, CYP 2E1 c2 and mEH exon 3 His alleles was statistically significantly associated with the occurrence of colorectal cancer
(OR: 1.44 95% CI: 1.04-2.00; OR: 1.74 95% CI: 1.15-2.65; OR: 1.79 95% CI: 1.10-2.92, respectively). Conclusion: These findings
suggest that allelic polymorphism of metabolizing enzymes play an important role in human colorectal carcinogenesis by affecting
the metabolism of dietary carcinogens.