Requirement of Tissue-Selective TBP-Associated Factor TAFII105 in Ovarian Development

• Published in: Science (American Association for the Advancement of Science) Vol. 293; no. 5537; pp. 2084 - 2087
• Main Authors: Freiman, Richard N., Albright, Shane R., Zheng, Shuang, Sha, William C., Hammer, Robert E., Tjian, Robert
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 14-09-2001; American Association for the Advancement of Science
• Subjects: Animals; B lymphocytes; Biological and medical sciences; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Down-Regulation; Female; Female animals; Follicles; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene Expression Regulation, Developmental; Genes; Germ cells; Granulosa cells; Granulosa Cells - metabolism; Granulosa Cells - physiology; In Situ Hybridization; Infertility, Female; Male; Mammalian female genital system; Mating behavior; Mice; Mice, Knockout; Morphology. Physiology; Oligonucleotide Array Sequence Analysis; Organ Size; Organ Specificity; Ovarian Follicle - growth & development; Ovaries; Ovary - cytology; Ovary - growth & development; Ovary - metabolism; Ovary - physiology; Ovulation; Protein Subunits; RNA; Signal Transduction; TATA-Binding Protein Associated Factors; Transcription Factor TFIID; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription Factors, TFII - metabolism; Transcription, Genetic; Vertebrates: reproduction; Transcription; Enzyme; Folliculogenesis; Transferases; Rodentia; Tissue specificity; Subunit; DNA-directed RNA polymerase; Ovary; Nucleotidyltransferases; Vertebrata; Mammalia; Mouse; Female genital system; Development; Transcription factor TFIID
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1061935

Transcription factor TFIID, composed of TBP and TAFIIsubunits, is a central component of the RNA polymerase II machinery. Here, we report that the tissue-selective TAFII105 subunit of TFIID is essential for proper development and function of the mouse ovary. Female mice lacking TAFII105 are viable but infertile because of a defect in folliculogenesis correlating with restricted expression of TAFII105 in the granulosa cells of the ovarian follicle. Gene expression profiling has uncovered a defective inhibin-activin signaling pathway in TAFII105-deficient ovaries. Together, these studies suggest that TAFII105 mediates the transcription of a subset of genes required for proper folliculogenesis in the ovary and establishes TAFII105 as a cell type-specific component of the mammalian transcriptional machinery.