Survival in vitro of motoneurons identified or purified by novel antibody-based methods is selectively enhanced by muscle-derived factors

Motoneurons were identified in vitro by a new method using the SC1 monoclonal antibody. They constituted up to 30% of total neurons in cultures of whole spinal cord from 4.5-day chicken embryos, and survived for at least 5 days in the presence of muscle extract, but not in its absence. By contrast,...

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Published in:	Development (Cambridge) Vol. 111; no. 1; pp. 221 - 232
Main Authors:	Bloch-Gallego, E, Huchet, M, el M'Hamdi, H, Xie, F K, Tanaka, H, Henderson, C E
Format:	Journal Article
Language:	English
Published:	Cambridge The Company of Biologists Limited 01-01-1991 Company of Biologists
Subjects:	Animals Biological and medical sciences Cell Separation Cell Survival - physiology Cells, Cultured Chick Embryo Embryology: invertebrates and vertebrates. Teratology Experimental organogenesis Fibroblast Growth Factors - physiology Fundamental and applied biological sciences. Psychology Growth Substances - physiology Motor Neurons - physiology Motor Neurons - ultrastructure Muscle Proteins - physiology Nerve Growth Factors - physiology Nerve Tissue Proteins - physiology Organogenesis. Physiological fonctions Spinal Cord - cytology Embryonic development Embryo Spinal cord Central nervous system Motor neuron Survival Vertebrata Mixed cell culture Polypeptide Muscle Chicken Aves Growth factor
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Summary:	Motoneurons were identified in vitro by a new method using the SC1 monoclonal antibody. They constituted up to 30% of total neurons in cultures of whole spinal cord from 4.5-day chicken embryos, and survived for at least 5 days in the presence of muscle extract, but not in its absence. By contrast, other neurons and floor-plate cells survived without muscle-derived factors. Motoneurons were purified to homogeneity by âpanningâ on dishes coated with SC1 antibody; they developed rapidly even in the absence of other spinal cells. Concentrations of muscle extract required for half-maximal motoneuron survival were indistinguishable in pure and mixed cultures, suggesting that muscle-derived factors act directly on motoneurons. Other purified growth factors tested, including ciliary neurotrophic factor, did not have the survival-promoting activity of muscle.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0950-1991 1477-9129