Investigating biofilm production, coagulase and hemolytic activity in candida species isolated from denture stomatitis patients/Protez stomatitli hastalardan izole edilen candida turlerinde biofilm uretimi, koagulaz ve hemolitik aktivite arastirilmasi

• Published in: The Eurasian journal of medicine Vol. 43; no. 1; p. 27
• Main Authors: Yigit, Nimet, Aktas, Esin, Dagistan, Saadettin, Ayyildiz, Ahmet
• Format: Journal Article
• Language: English
• Published: Istanbul AVES 01-04-2011; Galenos Publishing House
• Subjects: Bacteria; Candida; Dentures; Diagnosis; Health aspects; Identification systems; Microbial mats; Microbiology; Physiological aspects; Plasma; Proteins; Stomatitis; Studies; Yeast; Turkey
• ISSN: 1308-8734; 1308-8742
• DOI: 10.5152/eajm.2011.06

Objective: Oral candidiasis, in the form of Candida-associated denture stomatitis, represents a common disease in a large percentage of denture wearers, and Candida albicans remains the most commonly isolated species. In this study, we aimed to evaluate biofilm production, coagulase and hemolytic activity of Candida species isolated from denture stomatitis patients. Materials and Methods: This study included 70 patients (31 female, 39 male). Forty-eight of the patients were found to have a positive culture. A total of 48 Candida isolates representing five species, C. albicans (n=17), C. glabrata (n=10), C. krusei (n=9), C. kefyr (n=7) and C. parapsilosis (n=5), were tested. Their coagulase activities were evaluated by a classical tube coagulase test with rabbit plasma. A blood plate assay on 3% enriched sheep blood Sabouraud-dextrose agar (SDA) was used to determine their in vitro hemolytic activities. Biofilm production was determined by a visual tube method. Results: Twenty-one Candida isolates exhibited coagulase activity, and the coagulase activities of the C. albicans (64.7%) isolates were higher than other species. C. albicans, C. glabrata, C. kefyr and C. krusei species demonstrated beta hemolysis. C. parapsilosis strains failed to demonstrate any hemolytic activities. Fifteen (88.0%) of the C. albicans strains were biofilm positive. Six (35.2%) of these strains were strongly positive, 8 (47.0%) C. albicans strains were moderately positive and 1 (5.8%) C. albicans strain was weakly positive. Sixteen (51.6%) of the non-albicans Candida strains were biofilm positive while 15 (48.3%) did not produce biofilms. Conclusion: The results of this present study indicate coagulase, hemolytic activity and biofilm production by Candida spp. isolated from patients with denture stomatitis. Investigations of these virulence factors might be helpful in gaining information about the possible virulence of oral Candida species related to denture stomatitis. Key Words: Candida species, Coagulase activity, Denture stomatitis Amac: Oral kandidiyazisin Candida ile iliskili olarak gelisen protez stomatiti sekli, protez kullananlarin buyuk bir kisminda gorulen yaygin bir hastaliktir ve Candida albicans en cok izole edilen turdur. Bu calismada, protez stomatitli hastalardan izole edilen Candida turlerinde biofilm uretimi, koagulaz ve hemolitik aktivite arastirilmasi amaclanmistir. Gerec ve Yontem: Bu calisma 31 kadin, 39 erkek toplam 70 hasta uzerinde yurutuldu. Hastalarin 48'inde kultur pozitif bulundu ve izole edilen 48 Candida susu 5 tur olarak tiplendirildi [(C. albicans (n=17), C. glabrata (n=10), C. krusei (n=9), C. kefyr (n=7), C. parapsilosis (n=5)]. Suslarin koagulaz aktivitesi tavsan plazmasi kullanilarak klasik tup koagulaz testi ile hemolitik aktiviteleri ise %3 glikoz ile zenginlestirilmis koyun kanli Sabouraud-dextrose agar (SDA) kullanilarak arastirilmistir. Biofilm uretimi tup adherans testi ile belirlenmistir. Bulgular: Yirmibir Candida izolatinda koagulaz uretimi belirlenmis ve koagulaz aktivitesi Candida albicans (%64.7) suslarinda digerlerinden daha yuksek bulunmu?tur. Candida albicans, Candida glabrata, Candida kefyr ve Candida krusei suslari beta hemoliz olusturmustur. Candida parapsilosis suslari hemolitik aktivite gostermemistir. On bes (%88.0) C. albicans susu biofilm olusturmus, bunlarin altisinda (%35.2) kuvvetli, sekizinde (%47.0) orta dereceli ve birinde (%5.8) zayif biofilm olusumu belirlenmistir. On alti (%51.6) non-albicans Candida turunde biofilm olusumu pozitif bulunurken, 15'inde (%48.3) biofilm uretimi belirlenememistir. Sonuc: Bu calisma sonucunda protez stomatitli hastalardan izole edilen Candida turlerinde koagulaz, hemolitik aktivite ve biofilm uretimi belirlenmistir. Bu faktorlerin arastirilmasi protez stomatit iliskili Candida turlerinin muhtemel virulanslari hakkinda bilgi vermesi acisindan faydali olabilir. Anahtar Kelimeler: Candida turleri, Koagulaz aktivitesi, Protez stomatiti