Chemical Defense against Herbivory in the Brown Marine Macroalga IPadina gymnospora/I Could Be Attributed to a New Hydrocarbon Compound

• Published in: Plants (Basel) Vol. 12; no. 5
• Main Authors: Pereira, Renato Crespo, Paradas, Wladimir Costa, de Carvalho, Rodrigo Tomazetto, de Lima Moreira, Davyson, Kelecom, Alphonse, Passos, Raoni Moreira Ferreira, Atella, Georgia Correa, Salgado, Leonardo Tavares
• Format: Journal Article
• Language: English
• Published: MDPI AG 01-02-2023
• Subjects: Hydrocarbons; Identification and classification; Instrument industry; Pharmaceutical industry; Plant metabolites; Properties; Brazil; United States; Japan
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12051073

Brown marine macroalga Padina gymnospora (Phaeophyceae, Ochrophyta) produces both secondary metabolites (phlorotannins) and precipitate calcium carbonate (CaCO[sub.3]—aragonite) on its surface as potential defensive strategies against herbivory. Here, we have evaluated the effect of natural concentrations of organic extracts (dichloromethane—DI; ethyl acetate—EA and methanol—ME, and three isolated fractions) and mineralized tissues of P. gymnospora as chemical and physical resistance, respectively, against the sea urchin Lytechinus variegatus through experimental laboratory feeding bioassays. Fatty acids (FA), glycolipids (GLY), phlorotannins (PH) and hydrocarbons (HC) were also characterized and/or quantified in extracts and fractions from P. gymnospora using nuclear magnetic resonance (NMR) and gas chromatography (GC) coupled to mass spectrometry (CG/MS) or GC coupled to flame ionization detector (FID) and chemical analysis. Our results showed that chemicals from the EA extract of P. gymnospora were significantly important in reducing consumption by L. variegatus, but the CaCO[sub.3] did not act as a physical protection against consumption by this sea urchin. An enriched fraction containing 76% of the new hydrocarbon 5Z,8Z,11Z,14Z-heneicosatetraene exhibited a significant defensive property, while other chemicals found in minor amounts, such as GLY, PH, saturated and monounsaturated FAs and CaCO[sub.3] did not interfere with the susceptibility of P. gymnospora to L. variegatus consumption. We suggest that the unsaturation of the 5Z,8Z,11Z,14Z-heneicosatetraene from P. gymnospora is probably an important structural characteristic responsible for the defensive property verified against the sea urchin.