Genomic Analysis of IKlebsiella pneumoniae/I ST258 Strain Coproducing KPC-2 and CTX-M-14 Isolated from Poultry in the Brazilian Amazon Region

• Published in: Antibiotics (Basel) Vol. 11; no. 12
• Main Authors: Valiatti, Tiago Barcelos, Cayô, Rodrigo, Santos, Fernanda Fernandes, Bessa-Neto, Francisco Ozório, Brandão Silva, Ramon Giovani, Veiga, Ruanita, de Nazaré Miranda Bahia, Márcia, Guerra, Lívia Maria Guimarães Dutra, Pignatari, Antônio Carlos Campos, de Oliveira Souza, Cintya, Brasiliense, Danielle Murici, Gales, Ana Cristina
• Format: Journal Article
• Language: English
• Published: MDPI AG 01-12-2022
• Subjects: Bacterial pneumonia; Drug resistance in microorganisms; Imipenem; Pneumonia; Brazil; United Kingdom
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics11121835

This study aimed to characterize a Klebsiella pneumoniae strain (KP411) recovered from the stool samples of poultry (Gallus gallus) in the Brazilian Amazon Region. The whole-genome sequencing of KP411 revealed the presence of an important arsenal of antimicrobial resistance genes to β-lactams (bla[sub.CTX-M-14], bla[sub.TEM-1B], bla[sub.KPC-2], bla[sub.SVH-11]), aminoglycosides [aph(3″)- Ib, aph(6)-Id, aph(3′)-Ia], sulfonamides (sul1, sul2), quinolones (oqxAB), fosfomycin (fosA[sup.KP]), and macrolides [mph(A)]. Furthermore, our analyses revealed that the KP411 strain belongs to the ST258 clonal lineage, which is one of the main epidemic clones responsible for the dissemination of KPC-2 worldwide. Our data suggest that food-producing animals may act as reservoirs of multidrug-resistant K. pneumoniae belonging to the ST258 clone, and, consequently, contribute to their dissemination to humans and the environment.