Accumulation of organic solutes and enzymatic activity in cut roses cultivada com produtos de acao fisiologica no Vale do Submedio Sao Francisco

• Published in: Ciência rural Vol. 49; no. 12
• Main Authors: de Lourdes Neres da Silva, Maria, Santos, Mariana Correia, de Melo Barbosa, Mayara Suzanne, Sabino, Joao Henrique Ferreira, Ribeiro, Hugo Leonardo Coelho, Ramos, Anamaria Ribeiro Pereira, da Silva, Marcelle Almeida, Beckmann-Cavalcante, Markilla Zunete
• Format: Journal Article
• Language: Spanish
• Published: Universidade Federal de Santa Maria 01-12-2019
• Subjects: Agricultural chemicals; Amino acids; Antioxidants (Nutrients); Cytokinins; Enzymes; Gibberellins; Growth regulators; Plant hormones; Proline; Saturated fatty acids; Superoxides
• ISSN: 0103-8478

The aim of the present study was to evaluate the accumulation of carbohydrates, protein, and proline as well as the activity of the antioxidant enzymes superoxide dismutase and catalase in the Ambiance cultivar of cut rose plants grown with the application of physiological effect products in the Sub-Middle Sao Francisco River Valley in Brazil. The experiment was performed under a mesh screen with 50% shading. The experimental design used randomized blocks with four repetitions and six treatments: T1) control (water); T2) boscalid; T3) pyraclostrobin; T4) boscalid + pyraclostrobin (T2 + T3); T5) fluxapyroxad + pyraclostrobin; T6) plant growth regulators 4-(indol-3-yl) butyric acid (IBA) + gibberellic acid ([GA.sub.3]) + kinetin; these treatments were applied every 15 days throughout the crop cycle. To determine the accumulation of solutes and enzymatic activity, 8 leaves was collected every 48 h. Leaves were immediately immersed in liquid nitrogen and frozen until further analysis in the laboratory. Results showed that the product combinations boscalid + pyraclostrobin and fluxapyroxad + pyraclostrobin as well as the plant growth regulators were the treatments with the most consistent responses throughout the evaluated cycle, providing a greater accumulation of solutes in rose leaves, as an osmotic adjustment strategy against stress from high temperatures, particularly when proline accumulation is observed. With regard to enzymatic activity, plant regulators showed more consistent results when compared with other treatments, increasing both superoxide dismutase and catalase activity. The marked accumulation of organic solutes and the high enzymatic activity, particularly of catalase, indicated that rose plants use such mechanisms as a defense against the region's high temperatures. Key words: Rosa spp., strobilurins, plant growth regulators, carbohydrates, antioxidant enzymes. Objetivou-se, com este estudo, avaliar o acumulo de carboidratos, proteina e prolina, bem como a atividade de enzimas antioxidantes superoxido dismutase e catalases em plantas de rosa de corte 'Ambiance' cultivadas sob aplicacao de produtos de efeitos fisiologicos no Vale do Submedio Sao Francisco. O experimento foi realizado em telado com 50% de sombreamento. O delineamento experimental foi em blocos casualizados com quatro repeticoes e seis tratamentos: T1) testemunha (agua); T2) boscalida; T3) piraclostrobina; T4) boscalida + piraclostrobina (T2 + T3); T5) fluxapiroxade + piraclostrobina; T6) regulador vegetal IBA + [GA.sub.3] + cinetina, aplicados a cada 15 dias durante todo o ciclo da cultura. Para a determinacao do acumulo de solutos e atividade enzimatica foram realizadas 8 coletas de folhas a cada 48 horas. As mesmas foram imediatamente imersas em nitrogenio liquido e congeladas para analise posterior em laboratorio. Os resultados demonstram que os produtos conjugados boscalida + piraclostrobina, fluxapiroxade + piraclostrobina e os reguladores vegetais foram aqueles com respostas mais consistentes ao longo do ciclo avaliado, propiciando maior acumulo de solutos nas folhas das roseiras, como estrategia de ajustamento osmotico diante do estresse por temperatura elevada, especialmente quando se observa o acumulo de prolina. Para a atividade enzimatica, os reguladores vegetais apresentam resultados mais regulares quando comparados aos demais tratamentos, aumentando a atividade da SOD e da CAT. O acumulo acentuado de solutos organicos junto com a elevada atividade enzimatica, especialmente da CAT, indicam que as roseiras utilizam tais mecanismos como defesa diante das temperaturas elevadas da regiao. Palavras-chave: Rosa spp., estrobilurinas, reguladores vegetais, carboidratos, enzimas antioxidantes.