Evaluation of chemical characteristics and correlation analysis with pulp browning of advanced selections of apples grown in Brazil/Avaliacao das caracteristicas quimicas e analises de correlacao com o escurecimento da polpa de selecoes avancadas de macas cultivadas no Brasil

In this research, the total phenolic content (TPC) and antioxidant capacity (FRAP and DPPH* assays) of pulps and peels of advanced selection of apples grown in Brazil were investigated. The correlation analyses between the activity of polyphenoloxidase enzyme (PPO), vitamin C content, total titratab...

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Published in:Acta scientiarum. Technology Vol. 39; no. 1; p. 103
Main Authors: Ercoli, Luciana, Barizao, Erica Oliveira, Boeing, Joana Schuelter, Kvitschal, Marcus Vinicius, Visentainer, Jesui Vergilio, de Cinque Almeida, Vitor
Format: Journal Article
Language:Spanish
Published: Universidade Estadual de Maringa 01-01-2017
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Summary:In this research, the total phenolic content (TPC) and antioxidant capacity (FRAP and DPPH* assays) of pulps and peels of advanced selection of apples grown in Brazil were investigated. The correlation analyses between the activity of polyphenoloxidase enzyme (PPO), vitamin C content, total titratable acidity, and color parameters were performed. The results indicated that the data differed significantly among the apple genotypes studied. The peels of the selection Epagri 170-91 and Epagri 17025 showed the highest TPC and antioxidant capacities. In addition, the pulps of the Epagri 170-91 presented the highest TPC and antioxidant capacities, the lowest enzymatic browning, highest amount of vitamin C and lowest enzymatic activity when compared with other genotypes. The TPC and antioxidant capacities were significantly correlated in all genotypes analyzed. High correlation values between enzymatic browning and factors that affect the apple color were also found in all analyzed pulps, except between enzymatic browning and TPC. The results demonstrated that the enzymatic browning and TPC, as well as the antioxidant capacity and chemical characteristics, vary considerably depending on the apple genotypes and fruit tissues analyzed. Keywords: Antioxidant capacity, phenolic compounds, enzymatic browning, polyphenoloxidase enzyme, apple genotypes. Neste trabalho, o conteudo de fenolicos totais (CFT) e a capacidade antioxidante (ensaios FRAP e DPPH*) de polpas e cascas de selecoes avancadas de maca cultivadas no Brasil foram investigados. Analises de correlacao entre a atividade da enzima polifenoloxidase (PPO), o teor de vitamina C, acidez titulavel e parametros de cor foram realizadas. Os resultados indicaram diferenca significativa entre os diferentes genotipos de maca estudados. As cascas das selecoes Epagri 170-91 e Epagri 170-25 apresentaram os maiores resultados para CFT e capacidade antioxidante. Alem disso, a polpa da selecao Epagri 170-91 apresentou os melhores resultados de CFT e capacidade antioxidante, alem do menor escurecimento enzimatico, maior teor de vitamina C e menor atividade enzimatica quando comparada com as polpas de outros genotipos. A capacidade antioxidante e o CFT foram significativamente correlacionados em todos os genotipos analisados. Valores elevados de correlacao entre escurecimento enzimatico e fatores que afetam a cor da maca tambem foram encontrados em todas as polpas analisadas, exceto entre escurecimento enzimatico e CFT. Os resultados demonstraram que o escurecimento enzimatico e o CFT, bem como a capacidade antioxidante a as caracteristicas quimicas, variam consideravelmente dependendo do genotipo de maca e da parte da fruta analisada. Palavras-chave: capacidade antioxidante, compostos fenolicos, escurecimento enzimatico, enzima polifenoloxidase, genotipo de maca.
ISSN:1806-2563
DOI:10.4025/actascitechnol.v39i1.27913