Sperm Incubation in Biggers–Whitten–Whittingham Medium Induces Capacitation-Related Changes in the Lizard ISceloporus torquatus/I

• Published in: Animals (Basel) Vol. 14; no. 9
• Main Authors: Sánchez-Rivera, Uriel Ángel, Cruz-Cano, Norma Berenice, Medrano, Alfredo, Álvarez-Rodríguez, Carmen, Martínez-Torres, Martín
• Format: Journal Article
• Language: English
• Published: MDPI AG 01-05-2024
• Subjects: Endangered species; Lectins; Spermatozoa; Wildlife conservation; Uruguay; Mexico
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani14091388

Sperm acquire the ability to fertilize the egg during their transit through the female reproductive tract. This process, known as sperm capacitation, is well recognized in mammals and can be accomplished under laboratory conditions using specialized media. However, it remains unknown whether this process occurs in lizards. In this study, we investigated sperm incubation under conditions that promote capacitation to determine if similar changes occurred in their sperm. Our sperm assessment revealed functional changes, such as modifications in movement and staining patterns, commonly observed in mammals. This suggests that sperm capacitation may occur in this group of animals. Understanding sperm physiology is crucial for developing assisted reproduction technologies to aid conservation efforts for threatened species. Sperm capacitation involves biochemical and physiological changes that enable sperm to fertilize the oocyte. It can be induced in vitro under controlled conditions that simulate the environment of the oviduct. While extensively studied in mammals, its approach in lizards remains absent. Understanding the mechanisms that ensure reproduction is essential for advancing the implementation of assisted reproductive technologies in this group. We aimed to perform a sperm analysis to determine if capacitation-related changes were induced after incubation with capacitating media. Fifteen males of Sceloporus torquatus were collected during the early stage of the reproductive season. The sperm were isolated from the seminal plasma and then diluted up to a volume of 150 μL using BWW medium to incubate with 5% CO[sub.2] at 30 °C for a maximum duration of 3 h. A fraction was retrieved hourly for ongoing sperm assessment. The sperm analysis included assessments of its motility, viability, the capacitation status using the chlortetracycline (CTC) assay, and the acrosome integrity with the lectin binding assay to detect changes during incubation. We found that total motility was maintained up to 2 h post incubation, after which it decreased. However, sperm viability remained constant. From that moment on, we observed a transition to a deeper and less symmetrical flagellar bending in many spermatozoa. The CTC assay indicated a reduction in the percentage of sperm showing the full (F) pattern and an increase in those exhibiting the capacitated (B) and reactive (RA) patterns, accompanied by an elevation in the percentage of damaged acrosomes as revealed by the lectin binding assay. In mammals, these changes are often associated with sperm capacitation. Our observations support the notion that this process may also occur in saurian. While sperm analysis is a valuable method for assessing certain functional changes, additional approaches are required to validate this process.