Role of [Na.sup.+]/[K.sup.+]-ATPase in natriuretic effect of prolactin in a model of cholestasis of pregnancy

• Published in: Biochemistry (Moscow) Vol. 82; no. 5; p. 632
• Main Authors: Abramicheva, P.A, Balakina, T.A, Bulaeva, O.A, Guseva, A.A, Lopina, O.D, Smirnova, O.V
• Format: Journal Article
• Language: English
• Published: Springer 01-05-2017
• Subjects: Cholestasis; Development and progression; Genetic aspects; Health aspects; Pregnancy; Prolactin
• ISSN: 0006-2979
• DOI: 10.1134/S000629791705011X

Participation of [Na.sup.+]/[K.sup.+]-ATPase in the natriuretic effect of prolactin in a cholestasis of pregnancy model was investigated. The [Na.sup.+]/[K.sup.+]-ATPase activity in rat kidney medulla, where active sodium reabsorption occurs, decreased in the model of cholestasis of pregnancy and other hyperprolactinemia types compared with intact animals. This effect was not connected with the protein level of [alpha]l- and [beta]-subunits of [Na.sup.+]/[K.sup.+]-ATPase measured by Western blotting in the kidney medulla. Decrease in [Na.sup.+]/[K.sup.+]-ATPase activity in the kidney cortex was not significant, as well as decrease in the quantity of mRNA and proteins of the al- and [beta]-subunits of [Na.sup.+]/[K.sup.+]-ATPase. There were no correlations between the [Na.sup.+]/[K.sup.+]-ATPase activity and sodium clearance, although sodium clearance increased significantly in the model of cholestasis of pregnancy and other hyperprolactinemia groups under conditions of stable glomerular filtration rate measured by creatinine clearance. We conclude that the [Na.sup.+]/[K.sup.+]-ATPase is not the only mediator of the natriuretic effect of prolactin in the model of cholestasis of pregnancy. DOI: 10.1134/S000629791705011X Keywords: [Na.sup.+]/[K.sup.+]-ATPase, prolactin, natriuresis, cholestasis of pregnancy, kidney, rat