A Miniature Biomedical Sensor for Rapid Detection of ISchistosoma japonicum/I Antibodies

• Published in: Biosensors (Basel) Vol. 13; no. 8
• Main Authors: Hu, Shengjie, Jiang, Xuecheng, Yang, Liang, Tang, Xue, Yang, Guofeng, Hu, Yuanyuan, Wang, Jie, Lu, Naiyan
• Format: Journal Article
• Language: English
• Published: MDPI AG 01-08-2023
• Subjects: Analysis; Antibodies; Atomic force microscopy; Care and treatment; Diagnosis; Medical screening; Methods; Risk factors; Sensors; Testing; Viral antibodies; China
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios13080831

Schistosomiasis, typically characterized by chronic infection in endemic regions, has the potential to affect liver tissue and pose a serious threat to human health. Detecting and screening for this disease early on is crucial for its prevention and control. However, existing methods encounter challenges such as low sensitivity, time-consuming processes, and complex sample handling. To address these challenges, we report a soluble egg antigen (SEA)-based functionalized gridless and meander-type AlGaN/GaN high electron mobility transistors (HEMT) sensor for the highly sensitive detection of antibodies to Schistosoma japonicum. Immobilization of the self-assembled membrane on the gate surface was verified using a semiconductor parameter analyzer, scanning electron microscope (SEM), and atomic force microscopy (AFM). The developed biosensor demonstrates remarkable performance in detecting anti-SEA, exhibiting a linear concentration range of 10 ng/mL to 100 μg/mL and a sensitivity of 0.058 mA/log (ng/mL). It also exhibits similar excellent performance in serum systems. With advantages such as rapid detection, high sensitivity, miniaturization, and label-free operation, this biosensor can fulfill the requirements for blood defense.