GENE EXPRESSION OF MTOR, MURF-1 AND MAFBX IN WISTAR RATS SUPPLEMENTED WITH WHEY PROTEINS/Expressao genica de MTOR, MURF-1 e MAFBX em ratos Wistar suplementados com Whey Proteins

• Published in: Revista brasileira de obesidade, nutrição e emagrecimento Vol. 16; no. 104; p. 907
• Main Authors: de Macedo, Marcos Roberto Campos, Navarro, Antonio Coppi, Marques, Raphael Furtado, Silva, Melaine Mont'Alverne Lawall, Vidal, Flavia Castello Branco, Santiago-Silva, Alanna Joselle, Amorim, Carlos Eduardo Neves, Navarro, Francisco
• Format: Journal Article
• Language: English
• Published: Instituto Brasileiro de Pesquisa e Ensino em Fisiologia do Exercicio. IBPEFEX 01-09-2022
• ISSN: 1981-9919; 1981-9919

To verify the effect of supplementation of different doses of Whey Proteins for 12 weeks on the gene expression of MTOR, MURF-1, MAFBX and on the alteration of the cellular diameter of the gastrocnemius muscle. Sample: 38 Wistar rats and supplemented for 12 weeks. The white portion of the right gastrocnemius muscle was embedded in Rna Latter solution and stored at -80 [degrees] C and subjected to real-time polymerase chain reaction analysis. The left gastrocnemius muscle was intended for histological analysis. Gene expression of MTOR mRNA had lower statistical significance in the groups of Whey 2, Whey 4, Whey 6 than in the control group, in the order of 100%, 97%, 96%, respectively. Likewise, the gene expression of MURF-1 mRNA had lower statistical significance in the groups of Whey 2, Whey 4, Whey 6 than the control group, in the order of 65%, 75%, 84%, respectively. In line, the gene expression MAFBX mRNA had lower statistical significance in the groups Whey 2, Whey 4, Whey 6 in relation to the control group, in the order of 99%, 97%, 99%, respectively. There was no statistical difference in the cellular area of the gastrocnemius muscle between the groups. Therefore, supplementation of Whey Proteins at doses of 2, 4, 6 g/kg/day for 12 weeks in sedentary animals was not able to increase the gene expression of MTOR mRNA, however, it was able to reduce the gene expression of MURF-1 mRNA, MAFBX mRNA. Key words: Whey Proteins. MTOR. MUFR-1. MAFBX. Gastrocnemius. Verificar o efeito da suplementacao de diferentes doses de Whey Proteins por 12 semanas na expressao genica de MTOR, MURF-1, MAFBX e na alteracao do diametro celular do musculo gastrocnemio. Amostra: 38 ratos Wistar e suplementados por 12 semanas. A porcao branca do musculo gastrocnemio direito foi embebida em solucao de Rna Latter e armazenada a -80[degrees]C e submetida a analise de reacao em cadeia da polimerase em tempo real. O musculo gastrocnemio esquerdo foi destinado a analise histologica. A expressao genica do mRNA MTOR teve menor significancia estatistica nos grupos Whey 2, Whey 4, Whey 6 do que no grupo controle, na ordem de 100%, 97%, 96%, respectivamente. Da mesma forma, a expressao genica do mRNA de MURF-1 teve menor significancia estatistica nos grupos Whey 2, Whey 4, Whey 6 do que no grupo controle, na ordem de 65%, 75%, 84%, respectivamente. Em linha, a expressao genica MAFBX mRNA teve menor significancia estatistica nos grupos Whey 2, Whey 4, Whey 6 em relacao ao grupo controle, na ordem de 99%, 97%, 99%, respectivamente. Nao houve diferenca estatistica na area celular do musculo gastrocnemio entre os grupos. Portanto, a suplementacao de Whey Proteins nas doses de 2, 4, 6 g/kg/dia por 12 semanas em animais sedentarios nao foi capaz de aumentar a expressao genica de MTOR mRNA, porem, foi capaz de reduzir a expressao genica de MURF--1 mRNA, mRNA MAFBX. Palavras-chave: Whey Proteins, MTOR, MUFR-1, MAFBX, Gastrocnemio.