Development and application of a SARS-CoV-2 colorimetric biosensor based on the peroxidase-mimic activity of [gamma]-Fe.sub.2O.sub.3 nanoparticles
A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic [gamma] Fe.sub.2O.sub.3 nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of [gamma] Fe.sub.2...
Saved in:
Published in: | Mikrochimica acta (1966) Vol. 188; no. 10 |
---|---|
Main Authors: | , , , |
Format: | Journal Article |
Language: | English |
Published: |
Springer
01-10-2021
|
Subjects: | |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic [gamma] Fe.sub.2O.sub.3 nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of [gamma] Fe.sub.2O.sub.3 nanoparticles, the color change of H.sub.2O.sub.2 included 3,3',5,5'-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained. Graphical abstract |
---|---|
ISSN: | 0026-3672 |
DOI: | 10.1007/s00604-021-04989-6 |