Development and application of a SARS-CoV-2 colorimetric biosensor based on the peroxidase-mimic activity of [gamma]-Fe.sub.2O.sub.3 nanoparticles

A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic [gamma] Fe.sub.2O.sub.3 nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of [gamma] Fe.sub.2...

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Bibliographic Details
Published in:Mikrochimica acta (1966) Vol. 188; no. 10
Main Authors: Büyüksünetçi, Yudum Tepeli, Çitil, Burak Ekrem, Tapan, Utku, Anik, Ülkü
Format: Journal Article
Language:English
Published: Springer 01-10-2021
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Summary:A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic [gamma] Fe.sub.2O.sub.3 nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of [gamma] Fe.sub.2O.sub.3 nanoparticles, the color change of H.sub.2O.sub.2 included 3,3',5,5'-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained. Graphical abstract
ISSN:0026-3672
DOI:10.1007/s00604-021-04989-6