Increasing glutathione formation by functional expression of the [gamma]-glutamylcysteine synthetase gene in Saccharomyces cerevisiae

Increasing glutathione formation by functional expression of the [gamma]-glutamylcysteine synthetase gene in Saccharomyces cerevisiae

A recombinant plasmid, pGMF, containing a γ-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, was constructed with a copper-resistance gene as the selection marker and was introduced into S. cerevisiae YSF-31. The glutathione content of the recombinant strain was 1.5-fold (13.1...

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Bibliographic Details
Published in:Biotechnology letters Vol. 26; no. 5; p. 415
Main Authors: Fan, Xiuying, He, Xiuping, Guo, Xuena, Qu, Na, Wang, Changlu, Zhang, Borun
Format: Journal Article
Language:English
Published: Dordrecht Springer Nature B.V 01-03-2004
Subjects:
Yeast
Online Access:Get full text
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Summary:A recombinant plasmid, pGMF, containing a γ-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, was constructed with a copper-resistance gene as the selection marker and was introduced into S. cerevisiae YSF-31. The glutathione content of the recombinant strain was 1.5-fold (13.1 mg g dry cells^sup -1^) of that in the host strain.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/B:BILE.0000018261.14427.e8