Various cell types in human atherosclerotic lesions express ICAM-1. Further immunocytochemical and immunochemical studies employing monoclonal antibody 10F3

• Published in: The American journal of pathology Vol. 140; no. 4; pp. 889 - 896
• Main Authors: Printseva, OYu, Peclo, MM, Gown, AM
• Format: Journal Article
• Language: English
• Published: Bethesda, MD ASIP 01-04-1992; American Society for Investigative Pathology
• Subjects: Adult; Antibodies, Monoclonal; Antigens, CD - metabolism; Arteriosclerosis - metabolism; Arteriosclerosis - pathology; Biological and medical sciences; Cardiology. Vascular system; Cell Adhesion Molecules - metabolism; Cell Aggregation; Female; Humans; Immunohistochemistry; Intercellular Adhesion Molecule-1; Male; Medical sciences; Middle Aged; Precipitin Tests; Human; Pathology; Cell line; Immunocytochemistry; Atherosclerosis; Cardiovascular disease; Monoclonal antibody
• ISSN: 0002-9440; 1525-2191

The specificity of monoclonal antibody 10F3, generated to smooth muscle cells isolated from fetal human aorta, has been further explored in a series of biological, biochemical, and immunocytochemical studies. In the first assay, it was found that 10F3 could inhibit aggregation of phytohemagglutinin (PHA)-induced lymphocytes in a manner comparable to that of antibody RR1/1, an anti-intercellular adhesion molecule 1 (ICAM-1) monoclonal antibody. In immunoprecipitation experiments followed by one-dimensional gel electrophoresis, both 10F3 and RR1/1 immunoprecipitated 90 kd proteins, with results suggesting that the two antibodies recognized different epitopes of the same molecule. A series of immunocytochemical studies on human atherosclerotic lesions was performed; using single-labeling techniques, 10F3-positive cells were found in the vessel wall and in lesions of virtually all specimens of fatty streaks and fibrous plaques. Using double-labeling techniques, 10F3-positive macrophages and 10F3-positive smooth muscle cells were found; however, there were also a significant number of non-smooth muscle, nonmacrophage 10F3-positive cells. These studies demonstrate that 10F3 identifies ICAM-1, and that this protein is expressed on a variety of cell types in human atherosclerotic lesions. ICAM-1 may represent a developmentally regulated protein that is expressed in fetal but not adult mesenchymal cells, but can be re-expressed in pathologic processes such as atherosclerosis.