Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach

• Published in: Pakistan journal of pharmaceutical sciences Vol. 28; no. 4 Suppl; pp. 1449 - 1456
• Main Authors: Chunlei, Liu, Weihong, Min, Jingsheng, Liu, Yan, Liu, Jingxi, Ma, Jingjing, Li
• Format: Journal Article
• Language: English
• Published: Pakistan Pakistan Journal of Pharmaceutical Sciences 01-07-2015
• Subjects: Botanical research; Corylus - chemistry; Hazelnuts; Methods; Plant biochemistry; Plant proteins; Properties; Proteomics; Proteomics - methods
• ISSN: 1011-601X

Total proteins of Changbai Mountain hazelnut were extracted and then enzymolysed in solution. The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified proteins were analyzed by bioinformatics, 303 proteins were identified, of which 237 proteins (78.2%) were mainly distributing in a range of 10-70 kDa and 85 proteins (28.1%) were around pI 5-6. Based on the biological process cellular component and molecular function, these proteins were classified by Gene Ontology, the results showed that 183 proteins (73.8%) had catalytic activity (e.g. oxidoreductase, kinase, peptidase, etc.), and 170 proteins (68.5%) had binding activity. The protein profile provided a valuable basis for further research of hazelnut proteins and opened up new research avenues related to the function of these proteins.