Isolation of Extracellular Vesicles (EVs) Using Size-Exclusion High-Performance Liquid Chromatography (SE-HPLC)

Extracellular vesicles (EVs), are membrane-bound nanoparticles of biological origin. These signature molecules of health and disease have raised remarkable attention of the biomedical arena due to its potential diagnostic and therapeutic applicability. Among the many different techniques available f...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2273; p. 189
Main Authors:	Dissanayake, Keerthie, Godakumara, Kasun, Fazeli, Alireza
Format:	Journal Article
Language:	English
Published:	United States 2021
Subjects:	Animals Cell Culture Techniques Chromatography, Gel - instrumentation Chromatography, Gel - methods Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Culture Media, Conditioned - chemistry Equipment Design Extracellular Vesicles - chemistry Humans Extracellular vesicles Size-exclusion high-performance liquid chromatography (SE-HPLC)
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Summary:	Extracellular vesicles (EVs), are membrane-bound nanoparticles of biological origin. These signature molecules of health and disease have raised remarkable attention of the biomedical arena due to its potential diagnostic and therapeutic applicability. Among the many different techniques available for EV isolation, size-exclusion chromatography (SEC) is widely accepted.In this chapter, we present a protocol of size-exclusion high-performance liquid chromatography (SE-HPLC) as a method of EV isolation. This method can be adapted as a low cost but a reliable and scalable method of EV isolation in those laboratories having access to the HPLC systems.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-1246-0_13