Mouse Immunization with Radioattenuated Yeast Cells of Paracoccidioides brasiliensis

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1625; p. 3
• Main Authors: do Nascimento Martins, Estefânia Mara, de Andrade, Antero Silva Ribeiro
• Format: Journal Article
• Language: English
• Published: United States 2017
• Subjects: Animals; Antibodies, Fungal - immunology; Cytokines - blood; Cytokines - metabolism; Enzyme-Linked Immunosorbent Assay; Fungal Vaccines - immunology; Gamma Rays; Immunization; Immunocompromised Host; Male; Mice; Microbial Viability - radiation effects; Paracoccidioides - immunology; Paracoccidioides - pathogenicity; Paracoccidioides - radiation effects; Paracoccidioidomycosis - genetics; Paracoccidioidomycosis - immunology; Paracoccidioidomycosis - metabolism; Paracoccidioidomycosis - microbiology; Vaccines, Attenuated - immunology; Virulence; Live vaccine; Attenuation; Paracoccidioidomycosis; Gamma irradiation; Immunization; Paracoccidioides brasiliensis
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-7104-6_1

Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent mycosis in Latin America, and currently there is no effective vaccine. The present chapter describes the methodology to obtain radioattenuated yeast cells of P. brasiliensis and a protocol to evaluate protective response elicited by this immunogen in experimental paracoccidioidomycosis. The radioattenuated yeast provides a valuable tool for immunological studies in experimental paracoccidioidomycosis and vaccine research.