Effect of Bodily Fluids from Honey Bee

• Published in: PloS one Vol. 9; no. 2; p. e89175
• Main Authors: De Smet, Lina, De Koker, Dieter, Hawley, Alyse K, Foster, Leonard J, De Vos, Paul, de Graaf, Dirk C
• Format: Journal Article
• Language: English
• Published: Public Library of Science 20-02-2014
• Subjects: Bees; Genes; Genetic aspects; Genetic transcription; Genomes; Genomics; Growth; Honeybee
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0089175

Paenibacillus larvae, the causal agent of American Foulbrood disease (AFB), affects honey bee health worldwide. The present study investigates the effect of bodily fluids from honey bee larvae on growth velocity and transcription for this Gram-positive, endospore-forming bacterium. It was observed that larval fluids accelerate the growth and lead to higher bacterial densities during stationary phase. The genome-wide transcriptional response of in vitro cultures of P. larvae to larval fluids was studied by microarray technology. Early responses of P. larvae to larval fluids are characterized by a general down-regulation of oligopeptide and sugar transporter genes, as well as by amino acid and carbohydrate metabolic genes, among others. Late responses are dominated by general down-regulation of sporulation genes and up-regulation of phage-related genes. A theoretical mechanism of carbon catabolite repression is discussed.