Rotenone induced gene expression of anti oxidant stress markers in PC12 cells

• Published in: Indian journal of clinical biochemistry Vol. 33; no. S1; p. S53
• Main Authors: Aravind, P, Hemalatha, N, Bulbule, Sarojini R, Devaraju, K.S
• Format: Journal Article
• Language: English
• Published: Springer 24-05-2022
• Subjects: Alzheimer's disease; Antioxidants; Cell death; Development and progression; Gene expression; Genes; Genetic research; Nervous system diseases; Proteins
• ISSN: 0970-1915

Oxidative stress has been implicated in the progression of Alzheimer's disease (AD), Parkinson's disease (PD) and other neurodegenerative diseases. The effects of oxidative stress on the expression of antioxidant markers would give more insights into the molecular mechanism of the neurodegenerative disorders. Hence the current study is focused on the expression profile of antioxidant markers in Pheochromocytoma of the rat adrenal medulla (PC12) cells with rotenone. The oxidative stress been induced with Rotenone in PC12 cells. The stress markers such as Catalase, and r-SOD, r-Nrf2 and r-Gadd153 genes are studied by employing real time-PCR and protein expression level of MnSOD & iNOS by western blotting. The results demonstrate that rotenone treatment selectively down regulates SOD (0.5 fold) and Nrf2 (0.5 fold) expression, whereas it up regulates catalase (threefold), Btg2 (twofold) and Gadd153 (fourfold) gene expression compared to control. Protein expressions of MnSOD (95%) and iNOS (70%) were also down regulated compared to control. In conclusion, Dysfunction of endoplasmic reticulum leads to cell death and selective down regulation of stress markers. Therefore, we propose that PC12 cells undergo ER dysfunction during rotenone induced oxidative stress and suppress the mitochondrial stress responsive proteins leading to the cell death.