Closterovirus associated with leafroll and stem pitting in grapevine

The etiology of Grapevine leafroll (GLR) and stem-pitting (GSP) diseases is still uncertain, and various kinds of virus (a potyvirus, closterovirus-like particles, small isometric particles) have at times been implicated. Using the techniques of immunosorbent electron microscopy and decoration, 25 d...

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Published in:Phytopathologia mediterranea Vol. 24; no. 1-2; pp. 110 - 113
Main Authors: Conti, M, Milne, R.G. (Consiglio Nazionale delle Ricerche, Turin (Italy). Istituto di Fitovirologia Applicata)
Format: Journal Article
Language:English
Published: Unione Fitopatologica Mediterranea 01-08-1985
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Summary:The etiology of Grapevine leafroll (GLR) and stem-pitting (GSP) diseases is still uncertain, and various kinds of virus (a potyvirus, closterovirus-like particles, small isometric particles) have at times been implicated. Using the techniques of immunosorbent electron microscopy and decoration, 25 different grapevines suffering from GLR or GSP were examined for the presence of the two closteroviruses grapevine virus A (GVA) and B (GVB): 12 GLR samples and 9 GSP samples contained both GVA and GVB; two GLR samples and one GSP sample contained GVB alone, while none contained GVA alone. No closterovirus particles were detected in three healthy controls and in one GSP sample. Attempts to infect Grapevine seedlings with GVA from Nicotiana clevelandii Gray by mechanical inoculation or grafting were negative, as were attempts to transmit GVB from grapevines to herbaceous hosts. L'eziologia delle ampelopatie note come accartocciamento fogliare (GLR) e legno riccio (GSP) è tuttora incerta, sebbene in viti affette dall'una o dall'altra malattia sia stata rilevata la presenza di diversi tipi di virus (un potyvirus, alcuni closterovirus e virus isodiametrici piccoli). Mediante le tecniche di microscopia elettronica immuno-adsorbente e decorazione sono stati controllati per la presenza dei due closterovirus «grapevine virus A» (GVA) e «grapevine virus B» (GVB) 25 diverse viti affette da GLR o da GSP: 12 piante con GLR e 9 con GSP sono risultate affette sia da GVA che GVB, 2 piante con GLR e 1 con GSP dal solo GVB, nessuna pianta dal solo GVA. Tre piante sane di controllo ed una affetta da GSP non contenevano particelle di closterovirus. Diversi tentativi effettuati per infettare con GVA giovani semenzali di Vite mediante inoculazione meccanica o innesto da Nicotiana clevelandii Gray sono stati negativi, cosi come altri tentativi per trasmettere GVB da Vite a piante erbacee.
Bibliography:866113288
H20
ISSN:0031-9465
1593-2095