Functional and immunocytochemical evidence for the expression and localization of the secretory pathway Ca²⁺-ATPase isoform 1 (SPCA1) in cerebellum relative to other Ca²⁺ pumps

• Published in: Journal of neurochemistry Vol. 103; no. 3; pp. 1009 - 1018
• Main Authors: Sepúlveda, M. Rosario, Berrocal, María, Marcos, Daniel, Wuytack, Frank, Mata, Ana M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-11-2007; Blackwell Publishing Ltd; Blackwell
• Subjects: Adrenals. Interrenals; Adrenomedullary hormones. Regulation; Animals; Biochemistry; Biochemistry and metabolism; Biological and medical sciences; Brain; Calcium - metabolism; calcium pumps; Calcium Signaling - physiology; Calcium-Transporting ATPases - metabolism; Cell Membrane - enzymology; Cellular biology; Central nervous system; cerebellum; Cerebellum - enzymology; Cytosol - metabolism; Endoplasmic Reticulum - metabolism; Endoplasmic Reticulum - ultrastructure; Fundamental and applied biological sciences. Psychology; Golgi Apparatus - metabolism; Golgi Apparatus - ultrastructure; Golgi complex and secretory pathway; Hogs; Immunohistochemistry; Manganese - metabolism; Membranes; Neurology; Neurons - enzymology; Protein Isoforms - metabolism; secretory pathway Ca2+‐ATPase 1; secretory pathway Ca²⁺-ATPase 1; Secretory Vesicles - metabolism; Secretory Vesicles - ultrastructure; Signal Transduction - physiology; Sus scrofa; Vertebrates: endocrinology; Vertebrates: nervous system and sense organs; Cerebellum; Calcium; Central nervous system; Encephalon; transporting ATPase; secretory pathway Ca2+-ATPase 1; Localization; Neurological disorder; Ungulata; Vesicle; Nervous system diseases; Granule neuron; Enzyme; Ion transport; Golgi complex and secretory pathway; calcium pumps; Pig; Vertebrata; Mammalia; Membrane pump; Plasma membrane; Hydrolases; Artiodactyla; Basket neuron; Ca
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1111/j.1471-4159.2007.04794.x

Membrane fractions of pig cerebellum show Ca²⁺-ATPase activdity and Ca²⁺ transport due to the presence of the secretory pathway Ca²⁺-ATPase (SPCA). The SPCA1 isoform shows a wide distribution in the neurons of pig cerebellum, where it is found in the Golgi complex of the soma of Purkinje, stellate, basket and granule cells, and also in more distal components of the secretory pathway associated with a synaptic localization such as in cerebellar glomeruli. The SPCA1 may be involved in loading the Golgi complex and the secretory vesicles of these specific neuronal cell types with Ca²⁺ and also Mn²⁺. This study of the cellular and subcellular localization of SPCA1 pumps relative to the sarco(endo) plasmic reticulum Ca²⁺-ATPase and plasma membrane Ca²⁺-ATPase pumps hints to a possible specific role of SPCA1 in controlling the luminal secretory pathway Ca²⁺ (or Mn²⁺) levels as well as the local cytosolic Ca²⁺ levels. In addition, it helps to specify the zones that are most vulnerable to Ca²⁺ and/or Mn²⁺ dyshomeostasis, a condition that is held responsible of an increasing number of neurological disorders.